Development of Ag NPs/allantoin loaded PCL/GEL electrospun nanofibers for topical wound treatment.

IF 2.3 4区医学 Q3 ENGINEERING, BIOMEDICAL

Journal of Biomaterials Applications Pub Date : 2023-11-01 Epub Date: 2023-10-31 DOI:10.1177/08853282231212605

Mohsen Doostmohammadi, Seyyed Vahid Niknezhad, Hamid Forootanfar, Younes Ghasemi, Elham Jafari, Mahboubeh Adeli-Sardou, Armin Amirsadeghi, Alieh Ameri

{"title":"Development of Ag NPs/allantoin loaded PCL/GEL electrospun nanofibers for topical wound treatment.","authors":"Mohsen Doostmohammadi, Seyyed Vahid Niknezhad, Hamid Forootanfar, Younes Ghasemi, Elham Jafari, Mahboubeh Adeli-Sardou, Armin Amirsadeghi, Alieh Ameri","doi":"10.1177/08853282231212605","DOIUrl":null,"url":null,"abstract":"<p><p>In the present study, the allantoin and silver nanoparticle (Ag NPs) loaded poly caprolactone/gelatin (PCL/GEL) nanofibers produced using electrospinning technique and their cyto-compatibility and wound healing activity were evaluated in vitro and in vivo. The SEM imaging revealed diameters of 278.8 ± 10 and 240.6 ± 12 nm for PCL/GEL/Ag NPs and PCL/GEL/Ag NPs/allantoin scaffolds. The Ag NPs entrapment into scaffolds was evaluated by FTIR analysis and EDX mapping. Both scaffolds containing Ag NPs and Ag NPs/allantoin exhibited valuable wound healing activity in Wistar rat animal model. The profound granulation tissue formation, high collagen deposition in coordination with low level of edema and inflammatory cells in Ag NPs/allantoin loaded scaffolds resulted in complete and mature re-epithelialization in giving the healing score (12 out of 12) equal to positive control group to the wounds treated with these scaffolds. It was concluded that the Ag NPs/allantoin loaded scaffolds regarding to their good antibacterial activity and excellent wound healing activity could be introduced as new effective wound dressing materials.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"692-706"},"PeriodicalIF":2.3000,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Biomaterials Applications","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1177/08853282231212605","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/10/31 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}

引用次数: 0

Abstract

In the present study, the allantoin and silver nanoparticle (Ag NPs) loaded poly caprolactone/gelatin (PCL/GEL) nanofibers produced using electrospinning technique and their cyto-compatibility and wound healing activity were evaluated in vitro and in vivo. The SEM imaging revealed diameters of 278.8 ± 10 and 240.6 ± 12 nm for PCL/GEL/Ag NPs and PCL/GEL/Ag NPs/allantoin scaffolds. The Ag NPs entrapment into scaffolds was evaluated by FTIR analysis and EDX mapping. Both scaffolds containing Ag NPs and Ag NPs/allantoin exhibited valuable wound healing activity in Wistar rat animal model. The profound granulation tissue formation, high collagen deposition in coordination with low level of edema and inflammatory cells in Ag NPs/allantoin loaded scaffolds resulted in complete and mature re-epithelialization in giving the healing score (12 out of 12) equal to positive control group to the wounds treated with these scaffolds. It was concluded that the Ag NPs/allantoin loaded scaffolds regarding to their good antibacterial activity and excellent wound healing activity could be introduced as new effective wound dressing materials.

查看原文本刊更多论文

用于局部伤口治疗的Ag-NPs/尿囊素负载PCL/GEL电纺纳米纤维的开发。

在本研究中，使用静电纺丝技术制备的尿囊素和银纳米颗粒（Ag-NPs）负载的聚己内酯/明胶（PCL/GEL）纳米纤维及其细胞相容性和伤口愈合活性在体外和体内进行了评估。扫描电镜成像显示PCL/GEL/Ag NPs和PCL/GEL/Ag NPs/尿囊素支架的直径分别为278.8±10和240.6±12nm。通过FTIR分析和EDX图谱评估Ag NPs包埋到支架中。含有Ag NPs和Ag NPs/尿囊素的支架在Wistar大鼠动物模型中都表现出有价值的伤口愈合活性。在Ag-NPs/尿囊素负载的支架中，深刻的肉芽组织形成、高胶原沉积与低水平的水肿和炎症细胞相协调，导致了完全成熟的上皮再形成，使用这些支架治疗的伤口的愈合得分（12分中的12分）与阳性对照组相等。结果表明，Ag-NPs/尿囊素负载支架具有良好的抗菌活性和良好的伤口愈合活性，可作为新型有效的伤口敷料材料引入。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Biomaterials Applications 工程技术-材料科学：生物材料

CiteScore

5.10

自引率

3.40%

发文量

144

审稿时长

1.5 months

期刊介绍： The Journal of Biomaterials Applications is a fully peer reviewed international journal that publishes original research and review articles that emphasize the development, manufacture and clinical applications of biomaterials. Peer-reviewed articles by biomedical specialists from around the world cover: New developments in biomaterials, R&D, properties and performance, evaluation and applications Applications in biomedical materials and devices - from sutures and wound dressings to biosensors and cardiovascular devices Current findings in biological compatibility/incompatibility of biomaterials The Journal of Biomaterials Applications publishes original articles that emphasize the development, manufacture and clinical applications of biomaterials. Biomaterials continue to be one of the most rapidly growing areas of research in plastics today and certainly one of the biggest technical challenges, since biomaterial performance is dependent on polymer compatibility with the aggressive biological environment. The Journal cuts across disciplines and focuses on medical research and topics that present the broadest view of practical applications of biomaterials in actual clinical use. The Journal of Biomaterial Applications is devoted to new and emerging biomaterials technologies, particularly focusing on the many applications which are under development at industrial biomedical and polymer research facilities, as well as the ongoing activities in academic, medical and applied clinical uses of devices.