Plasma Cell-Free DNA Is a Potential Biomarker for Diagnosis of Calcific Aortic Valve Disease.

IF 1.9 4区医学 Q3 CARDIAC & CARDIOVASCULAR SYSTEMS

Cardiology Pub Date : 2024-01-01 Epub Date: 2023-10-27 DOI:10.1159/000534229

Wangge Ma, Wei Zhang, Huahua Liu, Benheng Qian, Rongguang Lai, Zijun Yao, Yidong Wang, Yang Yan, Zuyi Yuan

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引用次数: 0

Abstract

Introduction: Calcific aortic valve disease (CAVD) is the third most common cardiovascular disease in aging populations. Despite a growing number of biomarkers having been shown to be associated with CAVD, a marker suitable for routine testing in clinical practice is still needed. Plasma cell-free DNA (cfDNA) has been suggested as a biomarker for diagnosis and prognosis in multiple diseases. In this study, we aimed to test whether cfDNA could be used as a biomarker for the diagnosis of CAVD.

Methods: Serum samples were collected from 137 diagnosed CAVD patients and 180 normal controls. The amount of cfDNA was quantified by amplifying a short fragment (ALU 115) and a long fragment (ALU 247) using quantitative real-time PCR. The cfDNA integrity (cfDI) was calculated as the ratio of ALU247 to ALU115. The association between CAVD and cfDI was evaluated using regression analysis.

Results: CAVD patients had increased ALU 115 fragments (median, 185.14 (416.42) versus 302.83 (665.41), p < 0.05) but a decreased value of cfDI (mean, 0.50 ± 0.25 vs. 0.41 ± 0.26, p < 0.01) in their serum when compared to controls. This difference was more dramatic in non-rheumatic CAVD patients (p < 0.001) versus rheumatic CAVD patients (no significant difference). Similarly, CAVD patients with bicuspid aortic valve (BAV) (p < 0.01) showed a greater difference than non-BAV CAVD patients (p < 0.05). Linear regression and logistic regression showed that cfDI was independently and significantly associated with the presence of CAVD (95% CI, 0.096 to 0.773, p < 0.05). The ROC assay revealed that cfDI combined with clinical characteristics had a better diagnostic value than cfDI alone (AUC = 0.6191, p < 0.001).

Conclusion: cfDI may be a potential biomarker for diagnosis of CAVD.

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血浆细胞游离DNA是诊断钙化性主动脉瓣疾病的潜在生物标志物。

背景：钙化性主动脉瓣病（CAVD）是老年人群中第三常见的心血管疾病。尽管越来越多的生物标志物已被证明与CAVD相关，但仍需要一种适合临床实践中常规检测的标志物。血浆无细胞DNA（cfDNA）已被认为是多种疾病诊断和预后的生物标志物。在本研究中，我们旨在测试cfDNA是否可以作为诊断CAVD的生物标志物。方法：收集137名诊断为CAVD的患者和180名正常对照的血清样本。通过使用qPCR扩增短片段（ALU115）和长片段（ALU247）来定量cfDNA的量。cfDNA完整性（cfDI）计算为ALU247与ALU115的比率。使用回归分析评估CAVD和cfDI之间的相关性。结果：CAVD患者的ALU 115片段增加（中位数为185.14（416.42）vs 302.83（665.41），P结论：cfDI可能是诊断CAVD的潜在生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cardiology 医学-心血管系统

CiteScore

3.40

自引率

5.30%

发文量

审稿时长

1.5 months

期刊介绍： ''Cardiology'' features first reports on original clinical, preclinical and fundamental research as well as ''Novel Insights from Clinical Experience'' and topical comprehensive reviews in selected areas of cardiovascular disease. ''Editorial Comments'' provide a critical but positive evaluation of a recent article. Papers not only describe but offer critical appraisals of new developments in non-invasive and invasive diagnostic methods and in pharmacologic, nutritional and mechanical/surgical therapies. Readers are thus kept informed of current strategies in the prevention, recognition and treatment of heart disease. Special sections in a variety of subspecialty areas reinforce the journal''s value as a complete record of recent progress for all cardiologists, internists, cardiac surgeons, clinical physiologists, pharmacologists and professionals in other areas of medicine interested in current activity in cardiovascular diseases.