Determination of proteins in different feeds using the in sacco method in comparison to the CNCPS method

IF 0.7 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Z. Mlyneková, Z. Formelová, M. Chrenková, M. Rajský, M. Poláĉiková
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Abstract

The aim of this study was to determine the correlations between the output parameters of the in sacco method and the CNCPS method (Cornell Net Carbohydrate and Protein System). Both methods were tested on 106 samples from the feeds classified into following categories: extracted meal and cakes, cereals, legumes, maize and alfalfa silage, other silages, DDGS (dried distiller’s grains with solubles), oilseeds and various hays. The effective degradability of crude protein (EDg CP) by the in sacco method varied depending on the type of feed from 44.97% (hays) to 82.59% (alfalfa silage). The lowest degradation parameter of rapidly degraded fraction (a=24.3%) and the highest potentially degraded fraction (b=70.52%) were found in oilseeds. Five nitrogen fractions (A, B 1 , B 2 , B 3 , C) were determined according to the CNCPS method. Fraction A (NPN – non-protein nitrogen) was different in examined feed with the highest in silages (44.95% of the total CP). Fraction B 2 represented in tested feeds the highest part of the total CP, (except for silages, legumes, and oilseeds). Fraction B 3 ranged from 1.96% (legumes) to 19.25% (hays) of the total CP. The correlation between EDg CP and soluble fractions was low (r=0.5464) in concentrate feeds, the correlation between EDg and soluble fractions was (r=0.6323) also low in forages.
用甘蔗法测定不同饲料中的蛋白质,并与CNCPS法进行比较
本研究的目的是确定甘蔗法和CNCPS法(康奈尔净碳水化合物和蛋白质系统)输出参数之间的相关性。这两种方法在106种饲料样品上进行了测试,这些饲料分为以下几类:提取粕和蛋糕、谷物、豆类、玉米和苜蓿青贮饲料、其他青贮饲料、DDGS(干酒糟和可溶物)、油籽和各种干草。不同饲料类型的青贮法对粗蛋白质(EDg CP)的有效降解率为44.97%(干草)~ 82.59%(苜蓿青贮)。油籽中快速降解组分的降解参数最低(a=24.3%),潜在降解组分的降解参数最高(b=70.52%)。采用CNCPS法测定了5个氮组分(A、b1、b2、b3、C)。A组分(NPN -非蛋白氮)不同,青贮饲料中最高,占总CP的44.95%。在试验饲料中,b2是总CP的最高部分(青贮饲料、豆类和油籽除外)。在精料中,EDg CP与可溶性组分的相关性较低(r=0.5464),在牧草中,EDg CP与可溶性组分的相关性也较低(r=0.6323)。
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来源期刊
Journal of Central European Agriculture
Journal of Central European Agriculture AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
1.40
自引率
14.30%
发文量
46
审稿时长
50 weeks
期刊介绍: - General agriculture - Animal science - Plant science - Environment in relation to agricultural production, land use and wildlife management - Agricultural economics and rural development
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