Toxicity Assessment and Selective Leaching Characteristics of Cu-Al-Ni Shape Memory Alloys in Biomaterials Applications

Shih-Hang Chang, Bor-Yann Chen, Jin-Xiang Lin
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引用次数: 2

Abstract

Background Cu-Al-Ni shape memory alloys (SMAs) possess two-way shape memory effects, superelasticity, and damping capacity. Nonetheless, Cu-Al-Ni SMAs remain promising candidates for use in biomedical applications, as they are more economical and machinable than other SMAs. Ensuring the biocompatibility of Cu-Al-Ni SMAs is crucial to their development for biomedical applications. Therefore, this study aimed to assess the toxicity of Cu-Al-Ni SMAs using a Probit dose–response model and augmented simplex design. Methods In this study, the effects of Cu2+, Al3+ and Ni2+ metal ions on bacteria (Escherichia coli DH5α) using Probit dose–response analysis and augmented simplex design to assess the actual toxicity of the Cu-Al-Ni SMAs. Results Extraction and repetition of Escherichia coli DH5α solutions with high Cu2+ ion concentrations and 30-hour incubation demonstrated that Escherichia coli DH5α was able to alter its growth mechanisms in response to toxins. Metal ions leached from Cu-Al-Ni SMAs appeared in a multitude of compositions with varying degrees of toxicity, and those appearing close to a saddle region identified in the contour plot of the augmented simplex model were identified as candidates for elevated toxicity levels. When the Cu-13.5Al-4Ni SMA plate was immersed in Ringer's solution, the selective leaching rate of Ni2+ ions far exceeded that of Cu2+ and Al3+. The number of Cu2+, Al3+ and Ni2+ ions leached from Cu-Al-Ni SMAs increased with immersion time; however, at higher ratios, toxicity interactions among the metal ions had the effect of gradually reducing overall toxicity levels with regard to Escherichia coli DH5α. Conclusions The quantities of Cu2+, Al3+ and Ni2+ ions leached from the Cu-13.5Al-4Ni SMA plate increased with immersion time, the toxicity interactions associated with these compositions reduced the actual toxicity to Escherichia coli DH5α.
Cu-Al-Ni形状记忆合金在生物材料中的毒性评价及选择性浸出特性
Cu-Al-Ni形状记忆合金(sma)具有双向形状记忆效应、超弹性和阻尼能力。尽管如此,Cu-Al-Ni sma仍然是生物医学应用的有希望的候选者,因为它们比其他sma更经济和可加工。确保Cu-Al-Ni sma的生物相容性对其生物医学应用的发展至关重要。因此,本研究旨在使用Probit剂量-反应模型和增强单形设计来评估Cu-Al-Ni sma的毒性。方法采用Probit剂量效应分析和增强单形设计,研究Cu2+、Al3+和Ni2+金属离子对大肠杆菌DH5α的影响,评价Cu-Al-Ni sma的实际毒性。结果高Cu2+浓度的大肠杆菌DH5α溶液的提取和重复培养30小时表明,大肠杆菌DH5α能够改变其对毒素的生长机制。从Cu-Al-Ni sma中浸出的金属离子出现在具有不同程度毒性的多种成分中,并且那些出现在增强单纯形模型等高线图中确定的鞍区附近的金属离子被确定为毒性水平升高的候选物。当将Cu-13.5Al-4Ni SMA板浸入林格溶液时,Ni2+离子的选择性浸出率远远超过Cu2+和Al3+。Cu-Al-Ni sma浸出的Cu2+、Al3+和Ni2+离子数量随着浸出时间的增加而增加;然而,在较高的比例下,金属离子之间的毒性相互作用具有逐渐降低大肠杆菌DH5α总体毒性水平的作用。结论Cu-13.5Al-4Ni SMA板中Cu2+、Al3+和Ni2+离子的浸出量随浸出时间的增加而增加,其毒性相互作用降低了对大肠杆菌DH5α的实际毒性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Applied Biomaterials & Biomechanics
Journal of Applied Biomaterials & Biomechanics 生物-材料科学:生物材料
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