THE EFFECT OF GLYCEROL-3-PHOSPHATE ON STORAGE OF CPD PRESERVED WHOLE BLOOD

Abstract

Michio Kawashima and Michio Tsubokura Japanese Red Cross Tokyo Metropolitan Blood Center Adenosine triphosphate (ATP) and 2,3-diphosphoglycerate (2,3-DPG) are closely related to the functions of erythrocytes. We studied the effect of glycerol-3-phosphate on the levels of ATP and 2,3-DPG in erythrocyte. A single bag of donor blood was equally divided into two parts and glycerol-3-phosphate was added to one part. The other part was used as a control. We measured ATP, 2,3-DPG, plasma glucose, plasma lactate and the osmotic resistance levels of erythrocyte to evaluate the function of erythrocytes. As the results of these analyses, in the part to which glycerol-3-phosphate was added, ATP level showed 78.7% after three weeks and it maintained high level of 74.4% after five weeks. 2,3-DPG level showed 33.2% after three weeks, showing high value as compared with that of the control. And plasma glucose reduced rapidly while plasma lactate raised correspondingly, indicating that high activity was maintained in glucose metabolism. The osmotic resistance of erythrocytes also maintained good condition even after five weeks. These data suggest that the addition of glycerol-3-phosphate for storage of CPD preserved whole blood is effective to maintaine the function of erythrocyte.