Preparation of the Materials for Regenerative Medicine from Biological Tissues/Organs by Using High Hydrostatic Pressure Decellularization Method

Abstract

Decellularization Method 岸田 晶夫 Akio KISHIDA Decellularized tissue is promising as an ideal scaffold for tissue engineering. Decellularization techniques are classified by the chemicals used, such as acid or alkaline treatment, detergent treatment, or enzymatic digestion, and the physical methods used, such as snap freezing and mechanical agitation. As another candidate for a new decellularization treatment, we have developed the high-hydrostatic pressure ( HHP ) method. The unique characteristics of the HHP method are the destruction of cell membranes, uniform treatment, and short treat-ment time. Subsequent washing of the treated tissue can produce a decellularized tissue that