Methods in Comprehensive Mass Spectrometry-Based Measurement of Sphingolipids

Abstract

Molecular analysis of lipids has essentially improved by introduction of multidimensional mass spectrometry. This important technique allows identification and quantification of complex sphingolipids, e.g. glycosphingolipids and ceramides, from diverse intestinal sources including liver and bowel. In the following a short overview is given concerning this important technical tool which facilitates molecular characterization of lipids in intestinal physiology and diseases.