S. Raharja, Y. P. Rahardjo, Samsudin, Khaswar Syamsu
{"title":"Aroma precursor enhancing in dried cocoa beans fermentation using enzyme and heat addition","authors":"S. Raharja, Y. P. Rahardjo, Samsudin, Khaswar Syamsu","doi":"10.3934/agrfood.2023037","DOIUrl":null,"url":null,"abstract":"Changes in amino acids and reducing sugars in cocoa beans during fermentation were investigated using a 3 × 3 full complete factorial design using different enzyme additions (cellulase, papain and control–no enzyme) and water bath temperatures (40, 45 and 50 ℃) as variables over three days of fermentation. Aroma precursors (reducing sugars and free amino acids) developed inside the bean by enzymatic mechanisms during fermentation are converted into volatile compounds such as pyrazines and aldehydes during roasting. This study aimed to improve the fermentation process of dried beans by adding acetic acid, heat and enzymes, because there is insufficient pulp for the ideal spontaneous fermentation process. Samples were analyzed for fermentation index, cut bean, reducing sugar amino acid composition and volatile aroma composition profile using headspace solid phase microextraction (HS-SPME) and gas chromatography mass spectrometry (GC-MS). The results showed that the fermentation index was significantly affected by the addition of enzymes and water temperature. Although amino acids rose to 200%–300%, the composition contained several acidic amino acids because the pH utilized less than 4. Adding cellulase enzymes increases the amount of reducing sugars and amino acids but does not result in the formation of various amino acids.","PeriodicalId":44793,"journal":{"name":"AIMS Agriculture and Food","volume":null,"pages":null},"PeriodicalIF":1.9000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"AIMS Agriculture and Food","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3934/agrfood.2023037","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Changes in amino acids and reducing sugars in cocoa beans during fermentation were investigated using a 3 × 3 full complete factorial design using different enzyme additions (cellulase, papain and control–no enzyme) and water bath temperatures (40, 45 and 50 ℃) as variables over three days of fermentation. Aroma precursors (reducing sugars and free amino acids) developed inside the bean by enzymatic mechanisms during fermentation are converted into volatile compounds such as pyrazines and aldehydes during roasting. This study aimed to improve the fermentation process of dried beans by adding acetic acid, heat and enzymes, because there is insufficient pulp for the ideal spontaneous fermentation process. Samples were analyzed for fermentation index, cut bean, reducing sugar amino acid composition and volatile aroma composition profile using headspace solid phase microextraction (HS-SPME) and gas chromatography mass spectrometry (GC-MS). The results showed that the fermentation index was significantly affected by the addition of enzymes and water temperature. Although amino acids rose to 200%–300%, the composition contained several acidic amino acids because the pH utilized less than 4. Adding cellulase enzymes increases the amount of reducing sugars and amino acids but does not result in the formation of various amino acids.
期刊介绍:
AIMS Agriculture and Food covers a broad array of topics pertaining to agriculture and food, including, but not limited to: Agricultural and food production and utilization Food science and technology Agricultural and food engineering Food chemistry and biochemistry Food materials Physico-chemical, structural and functional properties of agricultural and food products Agriculture and the environment Biorefineries in agricultural and food systems Food security and novel alternative food sources Traceability and regional origin of agricultural and food products Authentication of food and agricultural products Food safety and food microbiology Waste reduction in agriculture and food production and processing Animal science, aquaculture, husbandry and veterinary medicine Resources utilization and sustainability in food and agricultural production and processing Horticulture and plant science Agricultural economics.