Simultaneous isoquercitin and gallic acid production of Aspergillus niger on Triphala byproduct under solid state fermentation in packed-bed bioreactor

IF 1.9 Q2 AGRICULTURE, MULTIDISCIPLINARY
Pattarabhorn Pakaweerachat, Worasaung Klinthong, K. Ohtaguchi, T. Chysirichote
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Abstract

Triphala byproduct from hot-water extraction (TPB), which was a traditional process, was valorized by solid state fermentation in this research. Since the leftovers from the extraction contain high rutin and tannin contents, they were hydrolysable to isoquercitin and gallic acid, which were their monomers, respectively. Aspergillus niger, a producer of α-L-rhamnosidase and β-glucosidase, was cultured on the TPB to produce both isoquercitin and gallic acid, which were powerful antioxidants used in medical applications. The solid-state fermentation (SSF) was conducted in the three-layered packed-bed bioreactor aerated with humid air at different rates (0.1, 0.2 and 0.3 L/L/min or vvm). The highest isoquercitin and gallic acid production rates were found in the SSF, with 0.1 vvm at 1.14/h and 0.3 vvm at 3.12/h, respectively. The interaction of aeration rate and fermentation time significantly affected the fungal growth and the production of gallic acid, while the isoquercitin production was affected only by the fermentation time. Moreover, the differences of their production yields in different positions of bed along the height of bioreactor found to be useful to design the harvesting period of the fermentation products including isoquercitin or gallic acid or simultaneous isoquercitin and gallic acid. The results clearly showed that aeration, harvesting time, and position of the bioreactor were crucial in designing the process for isoquercitin, gallic acid, or both.
黑曲霉在填充床生物反应器固体发酵下对Triphala副产物同时产异槲皮素和没食子酸的研究
采用固态发酵的方法,对传统热水浸提工艺中Triphala副产物进行了酸化处理。由于提取后的余物含有较高的芦丁和单宁含量,它们可水解为异槲皮素和没食子酸,这分别是它们的单体。在TPB上培养产生α- l -鼠李糖苷酶和β-葡萄糖苷酶的黑曲霉,产生异槲皮素和没食子酸,这两种抗氧化剂都是医学上应用的强效抗氧化剂。在三层填充床生物反应器中以不同的曝气速率(0.1、0.2和0.3 L/L/min或vvm)进行固态发酵。异槲皮素和没食子酸的产率最高,分别为1.14/h时的0.1 vvm和3.12/h时的0.3 vvm。曝气量和发酵时间的交互作用显著影响真菌的生长和没食子酸的产量,而异槲皮素的产量仅受发酵时间的影响。此外,它们在不同床位沿生物反应器高度的产率差异可用于设计发酵产物的收获期,包括异槲皮素或没食子酸或同时异槲皮素和没食子酸。结果清楚地表明,曝气、收获时间和生物反应器的位置对设计异槲皮素、没食子酸或两者的工艺至关重要。
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来源期刊
AIMS Agriculture and Food
AIMS Agriculture and Food AGRICULTURE, MULTIDISCIPLINARY-
CiteScore
3.70
自引率
0.00%
发文量
34
审稿时长
8 weeks
期刊介绍: AIMS Agriculture and Food covers a broad array of topics pertaining to agriculture and food, including, but not limited to:  Agricultural and food production and utilization  Food science and technology  Agricultural and food engineering  Food chemistry and biochemistry  Food materials  Physico-chemical, structural and functional properties of agricultural and food products  Agriculture and the environment  Biorefineries in agricultural and food systems  Food security and novel alternative food sources  Traceability and regional origin of agricultural and food products  Authentication of food and agricultural products  Food safety and food microbiology  Waste reduction in agriculture and food production and processing  Animal science, aquaculture, husbandry and veterinary medicine  Resources utilization and sustainability in food and agricultural production and processing  Horticulture and plant science  Agricultural economics.
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