Escherichia coli and Listeria innocua stability in carrot juice preserved by high hydrostatic pressure

IF 1.9 Q2 AGRICULTURE, MULTIDISCIPLINARY

AIMS Agriculture and Food Pub Date : 2022-01-01 DOI:10.3934/agrfood.2022039

J. Nasiłowska, B. Sokołowska, M. Fonberg-Broczek

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引用次数: 3

Abstract

The effect of High Hydrostatic Pressure (HHP) on Escherichia coli and Listeria innocua in carrot juice was evaluated just after pressurization and during refrigerated storage for 14 days. Samples were processed with different variants of pressure (300,400,500) and time (1 min, 5 min, and 10 min). The number of bacteria in the populations was analyzed using plating count methods. Required 5 log reduction was achieved only for L. innocua strains starting from 400 MPa for 5 min. E. coli strains displayed resistance to pressure, and the maximum reduction achieved was 2 log CFU/mL for the harshest process parameters. Sublethal injuries in the bacterial population were observed for all tested strains. According to two standardized ISO methods, selective conventional agars, TBX, and ALOA were used in the storage test. Additionally, the Thin Agar Layer (TAL) method was applied. In both used methods, the possibilities of recovery were provided. The regeneration was observed exclusively for L. innocua strains. The recovery of sublethally injured cells on ALOA and TAL did not differ statistically in every strain. In turn, results obtained for E. coli suggest that TBX may underestimate the number of HHP-injured bacteria.

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高压静水保存胡萝卜汁中大肠杆菌和无害李斯特菌的稳定性

研究了高静水压力(HHP)对加压后和冷藏14 d胡萝卜汁中大肠杆菌和无害李斯特菌的影响。样品在不同的压力(300,400,500)和时间(1分钟，5分钟和10分钟)下处理。采用平板计数法分析菌落中细菌数量。从400 MPa开始，持续5分钟，只有L. innocua菌株达到了所需的5 log降低。大肠杆菌菌株表现出耐压性，在最苛刻的工艺参数下，最大降低量为2 log CFU/mL。在所有测试菌株中观察到细菌种群的亚致死损伤。根据两种标准化的ISO方法，采用选择性常规琼脂、TBX和ALOA进行贮藏试验。此外，采用薄琼脂层(TAL)法。在这两种方法中，提供了回收的可能性。仅在innocua菌株中观察到再生。亚致死损伤细胞在ALOA和TAL上的恢复在各菌株间无统计学差异。反过来，大肠杆菌的结果表明，TBX可能低估了hhp损伤细菌的数量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

AIMS Agriculture and Food AGRICULTURE, MULTIDISCIPLINARY-

CiteScore

3.70

自引率

0.00%

发文量

审稿时长

8 weeks

期刊介绍： AIMS Agriculture and Food covers a broad array of topics pertaining to agriculture and food, including, but not limited to:  Agricultural and food production and utilization  Food science and technology  Agricultural and food engineering  Food chemistry and biochemistry  Food materials  Physico-chemical, structural and functional properties of agricultural and food products  Agriculture and the environment  Biorefineries in agricultural and food systems  Food security and novel alternative food sources  Traceability and regional origin of agricultural and food products  Authentication of food and agricultural products  Food safety and food microbiology  Waste reduction in agriculture and food production and processing  Animal science, aquaculture, husbandry and veterinary medicine  Resources utilization and sustainability in food and agricultural production and processing  Horticulture and plant science  Agricultural economics.