Maturity effect on the antioxidant activity of leaves and fruits of Rhodomyrtus tomentosa (Aiton.) Hassk.

IF 1.9 Q2 AGRICULTURE, MULTIDISCIPLINARY

AIMS Agriculture and Food Pub Date : 2022-01-01 DOI:10.3934/agrfood.2022018

Evi Mintowati Kuntorini, L. Nugroho, Maryani, T. R. Nuringtyas

{"title":"Maturity effect on the antioxidant activity of leaves and fruits of Rhodomyrtus tomentosa (Aiton.) Hassk.","authors":"Evi Mintowati Kuntorini, L. Nugroho, Maryani, T. R. Nuringtyas","doi":"10.3934/agrfood.2022018","DOIUrl":null,"url":null,"abstract":"Rhodomyrtus tomentosa has been used as a traditional medicine for a long time in Southeast Asia countries and also in Indonesia. It is believed that the phytochemical content of its fruit at different levels of ripeness may affect its antioxidant activity. Therefore, this study aims to determine the antioxidant activity, phenols and flavonoids contents, and their distribution in the leaves and fruits of R. tomentosa at different levels of maturity. The antioxidant activity was determined using DPPH and FRAP analysis. Furthermore, the total flavonoid and phenolic contents were analyzed using the colorimetric and Folin–Ciocalteu methods. The distribution of secondary metabolites in the leaves or fruits tissues was determined using histochemical analysis. Antioxidant capacity was analyzed using DPPH and FRAP, and a comparable result was obtained. The highest antioxidant activity was observed in the green fruit with the value of 1419.75 ± 3.48 and 1367.59 ± 9.12 μmol TE/g DW for DPPH and FRAP, respectively. The highest TFC value observed in the young leaves and green fruits of the ethanol extract was 96.375 ± 3.96 and 95.731 ± 5.42 mg QE/g DW, respectively. The highest TPC was found in the red fruits 50.772 ± 7.46 mgAGE/g DW. The histochemical analysis provided a clear distribution of flavonoid and phenolic within the leaves and fruits. It was found that both compounds accumulated in the epidermis, mesophyll, vascular bundles, secretory cavity, and parenchyma midrib of the leaves, and also in the exocarp and mesocarp endocarp, secretory cavity, vascular bundles, seed, trichomes of the fruits. This study showed that young leaves and green fruits have a higher potency of being a natural source of antioxidants and flavonoid compounds","PeriodicalId":44793,"journal":{"name":"AIMS Agriculture and Food","volume":"78 1","pages":""},"PeriodicalIF":1.9000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"AIMS Agriculture and Food","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3934/agrfood.2022018","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 3

Abstract

Rhodomyrtus tomentosa has been used as a traditional medicine for a long time in Southeast Asia countries and also in Indonesia. It is believed that the phytochemical content of its fruit at different levels of ripeness may affect its antioxidant activity. Therefore, this study aims to determine the antioxidant activity, phenols and flavonoids contents, and their distribution in the leaves and fruits of R. tomentosa at different levels of maturity. The antioxidant activity was determined using DPPH and FRAP analysis. Furthermore, the total flavonoid and phenolic contents were analyzed using the colorimetric and Folin–Ciocalteu methods. The distribution of secondary metabolites in the leaves or fruits tissues was determined using histochemical analysis. Antioxidant capacity was analyzed using DPPH and FRAP, and a comparable result was obtained. The highest antioxidant activity was observed in the green fruit with the value of 1419.75 ± 3.48 and 1367.59 ± 9.12 μmol TE/g DW for DPPH and FRAP, respectively. The highest TFC value observed in the young leaves and green fruits of the ethanol extract was 96.375 ± 3.96 and 95.731 ± 5.42 mg QE/g DW, respectively. The highest TPC was found in the red fruits 50.772 ± 7.46 mgAGE/g DW. The histochemical analysis provided a clear distribution of flavonoid and phenolic within the leaves and fruits. It was found that both compounds accumulated in the epidermis, mesophyll, vascular bundles, secretory cavity, and parenchyma midrib of the leaves, and also in the exocarp and mesocarp endocarp, secretory cavity, vascular bundles, seed, trichomes of the fruits. This study showed that young leaves and green fruits have a higher potency of being a natural source of antioxidants and flavonoid compounds

查看原文本刊更多论文

成熟度对毛蕊红叶和果实抗氧化活性的影响Hassk。

在东南亚国家和印度尼西亚，毛蕊红作为一种传统药物已经使用了很长时间。研究认为，不同成熟度果实的植物化学成分可能影响其抗氧化活性。因此，本研究旨在测定不同成熟度毛蕊花叶和果实的抗氧化活性、酚类物质和黄酮类物质含量及其分布。采用DPPH和FRAP分析测定其抗氧化活性。采用比色法和福林-酚法分析了总黄酮和酚类物质的含量。用组织化学方法测定了次生代谢物在叶片和果实组织中的分布。用DPPH和FRAP分析了抗氧化能力，得到了相似的结果。DPPH和FRAP在青果中的抗氧化活性最高，分别为1419.75±3.48和1367.59±9.12 μmol TE/g DW。幼叶和青果的TFC值最高，分别为96.375±3.96 mg QE/g DW和95.731±5.42 mg QE/g DW。红色果实TPC最高，为50.772±7.46 mg /g DW。组织化学分析明确了黄酮类和酚类物质在叶和果实中的分布。结果表明，这两种化合物均富集于叶片的表皮、叶肉、维管束、分泌腔和薄壁中脉，以及果实的外果皮和中果皮、内果皮、分泌腔、维管束、种子和毛状体。这项研究表明，嫩叶和绿色果实作为抗氧化剂和类黄酮化合物的天然来源具有更高的效力

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

AIMS Agriculture and Food AGRICULTURE, MULTIDISCIPLINARY-

CiteScore

3.70

自引率

0.00%

发文量

审稿时长

8 weeks

期刊介绍： AIMS Agriculture and Food covers a broad array of topics pertaining to agriculture and food, including, but not limited to:  Agricultural and food production and utilization  Food science and technology  Agricultural and food engineering  Food chemistry and biochemistry  Food materials  Physico-chemical, structural and functional properties of agricultural and food products  Agriculture and the environment  Biorefineries in agricultural and food systems  Food security and novel alternative food sources  Traceability and regional origin of agricultural and food products  Authentication of food and agricultural products  Food safety and food microbiology  Waste reduction in agriculture and food production and processing  Animal science, aquaculture, husbandry and veterinary medicine  Resources utilization and sustainability in food and agricultural production and processing  Horticulture and plant science  Agricultural economics.