Role of inhibitor of apoptosis proteins in TRAIL-induced apoptosis of gastric cancer cells

Q4 Pharmacology, Toxicology and Pharmaceutics

中国药理学通报 Pub Date : 2013-01-01 DOI:10.3969/J.ISSN.1001-1978.2013.06.024

Ping Wu, Yan Cheng, Xu Dong Zhang, Xiao Zhu, Lin Jie Zhang

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引用次数: 0

Abstract

Aim To explore the role of inhibitor of apoptosis proteins(IAP) in regulating the sensitivity of gastric cancer cells to tumor necrosis factor-related apoptosis-inducing ligand(TRAIL)-induced apoptosis.Methods Apoptotic cells were determined by the propidium iodide method using flow cytometry.The activation of caspase-3 and PARP cleavage were conducted by Western blot analysis.Expressions of XIAP,Survivin,cIAP1 and cIAP2 before and after treatment with TRAIL were also measured by Western blot analysis.Results TRAIL induced apoptosis in gastric cancer cells.BGC-823 cells were more sensitive to TRAIL than SGC-7901 cells.Caspse-3 activation and PARP cleavage were detected early after exposure to TRAIL.IAP family members were constitutively overexpressed in the two cell lines.The expression of XIAP was significantly downregulated in BGC-823 cells,compared with that in SGC-7901 cells,after TRAIL treatment.The expression of Survivin and cIAP1 remained unchanged.The expression of cIAP2 was slightly lowered in the two cell lines.Conclusions TRAIL-induced apoptosis of gastric cancer cells appears to be determined at the level of the effector caspase-3.XIAP protects gastric cancer cells from TRAIL-indued apoptosis.

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凋亡抑制蛋白在trail诱导的胃癌细胞凋亡中的作用

目的探讨凋亡抑制蛋白(IAP)在调节胃癌细胞对肿瘤坏死因子相关凋亡诱导配体(TRAIL)诱导的凋亡敏感性中的作用。方法采用碘化丙啶法，流式细胞术检测凋亡细胞。Western blot检测caspase-3的激活和PARP的裂解。Western blot检测TRAIL治疗前后XIAP、Survivin、cIAP1、cIAP2的表达。结果TRAIL诱导胃癌细胞凋亡。BGC-823细胞对TRAIL的敏感性高于SGC-7901细胞。TRAIL暴露后早期检测到caspase -3激活和PARP切割。IAP家族成员在两种细胞系中组成性过表达。TRAIL处理后，与SGC-7901细胞相比，BGC-823细胞中XIAP的表达明显下调。Survivin和cIAP1的表达保持不变。两种细胞系中cIAP2的表达均略有降低。结论trail诱导的胃癌细胞凋亡似乎是在效应蛋白caspase-3水平上决定的。XIAP保护胃癌细胞免受trail诱导的凋亡。

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来源期刊

中国药理学通报 Pharmacology, Toxicology and Pharmaceutics-Pharmacology

CiteScore

0.80

自引率

0.00%

发文量

11201

期刊介绍： Chinese Pharmacological Bulletin (CPB) is an academic monthly magazine sponsored by the Chinese Association for Science and Technology, the Chinese Pharmacological Society, and Anhui Medical University. Publicly issued domestically and internationally, CN:34:1086/R,ISSN:1001-1978。 The inclusion status of Chinese Pharmaceutical Bulletin (CPB): 1. This journal has been identified as a core medical journal by the China Science Citation Database (CSCD) of the Document Information Center of the Chinese Academy of Sciences (since 1993, there are 909 journals in the core library); 2. Selected as a pharmaceutical core journal by the editor in chief of Peking University Library's "Overview of Chinese Core Journals" (Peking University Core), 1st to 8th editions (1243 scientific and technological journals, including 232 medical and health journals); 3. Designated by the Institute of Science and Technology Information of the Ministry of Science and Technology of China as a source journal for Chinese scientific and technological paper statistics (China Science and Technology Core Journal); 4. Other: Domestic: Chinese Academic Journal Abstracts, Chinese Basic Medical Abstracts, Chinese Pharmaceutical Abstracts, Chinese Biological Abstracts, Chinese Academic Journals (CD version), Wanfang Database, VIP Database, etc; International: Chemical Abstract (USA), Corrective Bulletin on Science and Technology, CBST (Japan), Biological Abstract (USA), Biochemical Abstract (USA), EMBASE/Excerpta Medica (Netherlands), Index Copernicus (Poland), Kunst and Wissen (Germany), WPRIM (World Health Organization Western Pacific Regional Medical Index), Scopus (Netherlands, 2019 CiteScore 0.9), A&I (Netherlands), Centre for Agriculture and Biosciences international (CAB international, UK), Biosis Previews (USA), etc.