Transport behavior of baicalin in PC12 cells

Q4 Pharmacology, Toxicology and Pharmaceutics

中国药理学通报 Pub Date : 2012-06-01 DOI:10.3969/J.ISSN.1001-1978.2012.06.024

Shuang Zhao, F. Lei, H. Li, Yugang Wang, Yushuang Chai, L. Du

引用次数: 1

Abstract

Aim To investigate the transport behavior of baicalin in PC12 cells and to explain the material basement of nervous system protection activity of baicalin.Methods PC12 cells were used as for research,and HPLC and MS were applied to detect the transport behavior of baicalin.Results When cells were treated by baicalin of 0.1 mg·L-1,the concentration of baicalin in cells reached a peak when incubating for 30 min and there was no baicalin left after 8 h;when the dosage of baicalin from 0.05 mg·L-1 to 0.4 mg·L-1 was in administration,intracellular baicalin concentration and administration dosage showed linear positive correlation;verapamil and nimodipine had effect on transport;in addition,baicalein and 6-methoxy-baicalin could be detected in the cells.Conclusion Baicalin can transport into PC12 cells and can be affected by some inhibitors,producing compounds.

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黄芩苷在PC12细胞中的转运行为

目的探讨黄芩苷在PC12细胞中的转运行为，探讨黄芩苷保护神经系统活性的物质基础。方法以PC12细胞为研究对象，采用高效液相色谱法和质谱法检测黄芩苷的转运行为。结果当细胞被0.1 mg·l - 1的黄芩苷,黄芩苷的浓度在细胞在孵化30分钟到达顶点,没有8 h后黄芩苷留下;当从0.05毫克的剂量黄芩苷·l - 1到0.4 mg·l - 1公司在管理、胞内黄芩苷浓度和管理剂量显示线性正相关;维拉帕米和nimodipine影响运输;此外,黄芩黄素和6-methoxy-baicalin可以检测到细胞。结论黄芩苷可转运至PC12细胞，并可受某些抑制剂的影响而产生化合物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

中国药理学通报 Pharmacology, Toxicology and Pharmaceutics-Pharmacology

CiteScore

0.80

自引率

0.00%

发文量

11201

期刊介绍： Chinese Pharmacological Bulletin (CPB) is an academic monthly magazine sponsored by the Chinese Association for Science and Technology, the Chinese Pharmacological Society, and Anhui Medical University. Publicly issued domestically and internationally, CN:34:1086/R,ISSN:1001-1978。 The inclusion status of Chinese Pharmaceutical Bulletin (CPB): 1. This journal has been identified as a core medical journal by the China Science Citation Database (CSCD) of the Document Information Center of the Chinese Academy of Sciences (since 1993, there are 909 journals in the core library); 2. Selected as a pharmaceutical core journal by the editor in chief of Peking University Library's "Overview of Chinese Core Journals" (Peking University Core), 1st to 8th editions (1243 scientific and technological journals, including 232 medical and health journals); 3. Designated by the Institute of Science and Technology Information of the Ministry of Science and Technology of China as a source journal for Chinese scientific and technological paper statistics (China Science and Technology Core Journal); 4. Other: Domestic: Chinese Academic Journal Abstracts, Chinese Basic Medical Abstracts, Chinese Pharmaceutical Abstracts, Chinese Biological Abstracts, Chinese Academic Journals (CD version), Wanfang Database, VIP Database, etc; International: Chemical Abstract (USA), Corrective Bulletin on Science and Technology, CBST (Japan), Biological Abstract (USA), Biochemical Abstract (USA), EMBASE/Excerpta Medica (Netherlands), Index Copernicus (Poland), Kunst and Wissen (Germany), WPRIM (World Health Organization Western Pacific Regional Medical Index), Scopus (Netherlands, 2019 CiteScore 0.9), A&I (Netherlands), Centre for Agriculture and Biosciences international (CAB international, UK), Biosis Previews (USA), etc.