Geroprotective effects of ×Sorbaronia mitschurinii fruit extract on Drosophila melanogaster

IF 1.5 4区农林科学 Q3 PLANT SCIENCES

Journal of Berry Research Pub Date : 2021-10-26 DOI:10.3233/jbr-211502

E. Platonova, N. Zemskaya, M. Shaposhnikov, D. Golubev, Daria V. Kukuman, Natalya R. Pakshina, N. Ulyasheva, V. Punegov, S. Patov, A. Moskalev

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引用次数: 2

Abstract

BACKGROUND: ×Sorbaronia mitschurinii (also known as Aronia mitschurinii) is an intergeneric hybrid of×Sorbaronia fallax and Aronia melanocarpa. ×S. mitschurinii berry is a rich source of phytochemicals such as flavonoids and anthocyanins, which have a broad range of health benefits and a great geroprotective potential. OBJECTIVES: The goal of the present study was to investigate the geroprotective effects of Sorbaronia berry ethanolic extract (SBE) in Drosophila melanogaster and whether these effects depend on the concentration of SBE, duration of treatment, age, and sex. METHODS: SBE was used to supplement a diet of Drosophila imagoes throughout life, during 2 weeks after the imago hatching, and from 4 to 6 weeks of age. The relationship between the SBE effects on lifespan, stress resistance and the expression of stress response genes were examined. RESULTS: SBE treatment at 1-2 and 4-6 weeks of life increased the lifespan, while treatment throughout life reduced lifespan. SBE treatment increased Drosophila resistance to oxidative stress and starvation, but not to hyperthermia. A statistically significant effect of SBE treatment on the expression level of per, keap1, hif1, hsp27, hsp68, hsp83, and sirt1 genes in flies of both sexes and on sod1 expression only in female flies was demonstrated. CONCLUSIONS: The observed relationship between SBE concentration and lifespan effects suggests that the life-extending effect of SBE may be associated with the mechanism of hormesis.

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×Sorbaronia米氏果提取物对黑腹果蝇的保护作用

背景:×Sorbaronia mitschurinii(也被称为Aronia mitschurinii)是一个属间杂交of×Sorbaronia黄豆和黑桫椤。×S。蓝莓含有丰富的植物化学物质，如黄酮类化合物和花青素，具有广泛的健康益处和巨大的保护潜力。目的:本研究的目的是研究Sorbaronia berry乙醇提取物(SBE)对果蝇的衰老保护作用，以及这些作用是否取决于SBE的浓度、治疗时间、年龄和性别。方法:SBE在果蝇成虫一生中、成虫孵化后2周和4 ~ 6周龄期间补充果蝇的饮食。研究了SBE对寿命、抗逆性和应激反应基因表达的影响。结果:SBE治疗在1-2周和4-6周增加了寿命，而整个生命周期治疗减少了寿命。SBE治疗增加了果蝇对氧化应激和饥饿的抵抗力，但没有增加对高温的抵抗力。SBE处理对两性果蝇中per、keap1、hif1、hsp27、hsp68、hsp83和sirt1基因的表达水平以及仅对雌性果蝇中sod1的表达有统计学意义。结论:观察到SBE浓度与寿命效应的关系，提示SBE的延寿作用可能与激效机制有关。

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来源期刊

Journal of Berry Research Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

3.50

自引率

11.80%

发文量

期刊介绍： The main objective of the Journal of Berry Research is to improve the knowledge about quality and production of berries to benefit health of the consumers and maintain profitable production using sustainable systems. The objective will be achieved by focusing on four main areas of research and development: From genetics to variety evaluation Nursery production systems and plant quality control Plant physiology, biochemistry and molecular biology, as well as cultural management Health for the consumer: components and factors affecting berries'' nutritional value Specifically, the journal will cover berries (strawberry, raspberry, blackberry, blueberry, cranberry currants, etc.), as well as grapes and small soft fruit in general (e.g., kiwi fruit). It will publish research results covering all areas of plant breeding, including plant genetics, genomics, functional genomics, proteomics and metabolomics, plant physiology, plant pathology and plant development, as well as results dealing with the chemistry and biochemistry of bioactive compounds contained in such fruits and their possible role in human health. Contributions detailing possible pharmacological, medical or therapeutic use or dietary significance will be welcomed in addition to studies regarding biosafety issues of genetically modified plants.