Development, validation, and application of UPLC-PDA method for anthocyanins profiling in Vaccinium L. berries

IF 1.5 4区农林科学 Q3 PLANT SCIENCES

Journal of Berry Research Pub Date : 2021-01-03 DOI:10.3233/jbr-200658

G. Vilkickytė, V. Motiekaitytė, R. Vainorienė, M. Liaudanskas, L. Raudonė

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引用次数: 15

Abstract

BACKGROUND: Anthocyanins are one of the key factors contributing to the quality and biological activities in various berries. Particular attention was devoted to Vaccinium L. berries, because of being commonly consumed in daily life and providing a large potential for the development of new pharmaceutical applications. OBJECTIVE: The present work aimed at establishing a novel UPLC-PDA method for profiling anthocyanins in berries and evaluating their distribution in cultivated lingonberries. METHODS: The method was developed and validated using bilberries (Vaccinium myrtillus L.), cranberries (Vaccinium macrocarpon Ait.), and lingonberries (Vaccinium vitis-idaea L.) matrices. Anthocyanins content variation was analyzed among 7 different cultivars and 1 infraspecific taxon of lingonberries. RESULTS: The proposed method ensured the separation of 20 compounds, including major and minor anthocyanins and their aglycones, in bilberries, 15 in cranberries, and 9 in lingonberries, during 12 min analysis. Bilberry matrix was distinguished by the most complex profile and the presence of delphinidin and petunidin, which were not identified either in cranberries or lingonberries. Cranberry anthocyanins consisted mainly of the 3-galactosides of cyanidin and peonidin, whereas lingonberry anthocyanins included cyanidin and its glycosides with just traces of other anthocyanins. Obtained anthocyanins fingerprinting results have guidance function in practice and demonstrate valuable chemophenetic information for studied Vaccinium berries. All determined method validation values were considered to be acceptable. Variation analysis of anthocyanins levels among cultivated lingonberries suggested genetic diversity and because of the highest anthocyanins content—the superiority of Russian cultivars (‘Kostromička’ and ‘Rubin’). To the best of our knowledge, this is the first comprehensive report on the anthocyanins of certain lingonberry cultivars. CONCLUSIONS: This study resulted in the rapid, simple, and validated method, which was shown to be applicable and convenient for routine analysis and authentication of Vaccinium berry samples.

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UPLC-PDA方法的建立、验证及应用

背景:花青素是影响各种浆果品质和生物活性的关键因素之一。由于在日常生活中被广泛食用，并且为开发新的药物应用提供了巨大的潜力，因此特别关注了Vaccinium L.浆果。目的:建立一种新的UPLC-PDA方法来分析越橘果实中花青素的含量，并评价其在栽培越橘中的分布。方法:以越桔(Vaccinium myrtillus L.)、蔓越莓(Vaccinium macrocarpon Ait.)和越橘(Vaccinium vitis- idea L.)为基质，建立并验证了该方法。分析了越橘7个不同品种和1个种下分类群的花色苷含量差异。结果:该方法在12 min的分析时间内，保证了越桔、蔓越莓和越橘中20个化合物(主要和次要花青素及其苷元)、15个化合物(次要花青素及其苷元)和9个化合物(次要花青素及其苷元)的分离。越桔基质以其最复杂的结构特征和在蔓越莓和越橘中均未发现的飞燕草苷和矮缩草苷的存在来区分。蔓越莓花青素主要由花青素和芍药苷的3-半乳糖苷组成，而越橘花青素包括花青素及其苷类，仅含有少量其他花青素。所获得的花青素指纹图谱结果具有实际指导作用，为所研究的越橘提供了有价值的化学化学信息。所有确定的方法验证值都被认为是可接受的。栽培越橘中花色苷含量的变异分析表明，越橘具有遗传多样性，花色苷含量最高的品种是俄罗斯品种(kostromi ka和Rubin)。据我们所知，这是第一份关于某些越橘品种花青素的综合报告。结论:本研究建立的方法快速、简便、有效，适用于枸杞样品的常规分析和鉴定。

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来源期刊

Journal of Berry Research Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

3.50

自引率

11.80%

发文量

期刊介绍： The main objective of the Journal of Berry Research is to improve the knowledge about quality and production of berries to benefit health of the consumers and maintain profitable production using sustainable systems. The objective will be achieved by focusing on four main areas of research and development: From genetics to variety evaluation Nursery production systems and plant quality control Plant physiology, biochemistry and molecular biology, as well as cultural management Health for the consumer: components and factors affecting berries'' nutritional value Specifically, the journal will cover berries (strawberry, raspberry, blackberry, blueberry, cranberry currants, etc.), as well as grapes and small soft fruit in general (e.g., kiwi fruit). It will publish research results covering all areas of plant breeding, including plant genetics, genomics, functional genomics, proteomics and metabolomics, plant physiology, plant pathology and plant development, as well as results dealing with the chemistry and biochemistry of bioactive compounds contained in such fruits and their possible role in human health. Contributions detailing possible pharmacological, medical or therapeutic use or dietary significance will be welcomed in addition to studies regarding biosafety issues of genetically modified plants.