Transcriptome-based identification of the beneficial role of blackcurrant, strawberry and yellow onion to attenuate the cytopathic effects of Clostridium difficile toxins

IF 1.5 4区农林科学 Q3 PLANT SCIENCES

Journal of Berry Research Pub Date : 2021-06-14 DOI:10.3233/JBR-200646

Prashanna Balaji Venkatasubramanian, Els Oosterink, M. Tomassen, M. Suárez-Diez, J. Mes, E. Saccenti, N. Wit

{"title":"Transcriptome-based identification of the beneficial role of blackcurrant, strawberry and yellow onion to attenuate the cytopathic effects of Clostridium difficile toxins","authors":"Prashanna Balaji Venkatasubramanian, Els Oosterink, M. Tomassen, M. Suárez-Diez, J. Mes, E. Saccenti, N. Wit","doi":"10.3233/JBR-200646","DOIUrl":null,"url":null,"abstract":"BACKGROUND: Clostridium difficile Infection (CDI) can lead to diarrhea and fulminant colitis. C. difficile infects the host using toxins. Recent studies report prevalence of CDI in the small intestine. Berries are known to contain antioxidants and phenolic compounds that might mitigate bacterial infection. OBJECTIVE: We explored the impact of C. difficile toxins on the small intestine using an in vitro approach and used systems biology techniques together with data integration to identify food compounds that can reduce their cytopathic impact. METHODS: Differentiated Caco-2 cells were exposed to C. difficile toxins and the transcriptomic changes were studied. To identify foods with potential beneficial counteracting effects, the transcriptomic profiles were integrated with transcriptomics data from Caco-2 cells exposed to various food compounds and analyzed using multivariate analysis. RESULTS: Beneficial food candidates, selected by multivariate analysis, such as blackcurrant, strawberry and yellow onion were further examined for their potential to counteract the effect of the toxin-induced disruption of cell integrity and toxin translocation. Our results confirmed effects of food compounds, on the cytopathic effects of toxins in the small intestine. CONCLUSION: Blackcurrant, strawberry and yellow onion can counteract C. difficile toxins induced effects.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":"11 1","pages":""},"PeriodicalIF":1.5000,"publicationDate":"2021-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/JBR-200646","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Berry Research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.3233/JBR-200646","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 2

Abstract

BACKGROUND: Clostridium difficile Infection (CDI) can lead to diarrhea and fulminant colitis. C. difficile infects the host using toxins. Recent studies report prevalence of CDI in the small intestine. Berries are known to contain antioxidants and phenolic compounds that might mitigate bacterial infection. OBJECTIVE: We explored the impact of C. difficile toxins on the small intestine using an in vitro approach and used systems biology techniques together with data integration to identify food compounds that can reduce their cytopathic impact. METHODS: Differentiated Caco-2 cells were exposed to C. difficile toxins and the transcriptomic changes were studied. To identify foods with potential beneficial counteracting effects, the transcriptomic profiles were integrated with transcriptomics data from Caco-2 cells exposed to various food compounds and analyzed using multivariate analysis. RESULTS: Beneficial food candidates, selected by multivariate analysis, such as blackcurrant, strawberry and yellow onion were further examined for their potential to counteract the effect of the toxin-induced disruption of cell integrity and toxin translocation. Our results confirmed effects of food compounds, on the cytopathic effects of toxins in the small intestine. CONCLUSION: Blackcurrant, strawberry and yellow onion can counteract C. difficile toxins induced effects.

查看原文本刊更多论文

基于转录组的黑加仑、草莓和黄洋葱减轻艰难梭菌毒素细胞病变作用的有益鉴定

背景:艰难梭菌感染(CDI)可导致腹泻和暴发性结肠炎。艰难梭菌利用毒素感染宿主。最近的研究报告了小肠中CDI的患病率。众所周知，浆果含有抗氧化剂和酚类化合物，可以减轻细菌感染。目的:我们使用体外方法探索艰难梭菌毒素对小肠的影响，并使用系统生物学技术和数据整合来识别可以减少其细胞病变影响的食物化合物。方法:将分化后的Caco-2细胞暴露于艰难梭菌毒素中，观察其转录组学变化。为了确定具有潜在有益抵消作用的食物，将转录组学数据与暴露于各种食物化合物的Caco-2细胞的转录组学数据相结合，并使用多变量分析进行分析。结果:通过多变量分析选择了有益的候选食物，如黑加仑、草莓和黄洋葱，进一步研究了它们抵消毒素引起的细胞完整性破坏和毒素易位影响的潜力。我们的结果证实了食物化合物对小肠中毒素的细胞病变效应的影响。结论:黑加仑、草莓、黄洋葱均可拮抗艰难梭菌毒素的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Berry Research Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

3.50

自引率

11.80%

发文量

期刊介绍： The main objective of the Journal of Berry Research is to improve the knowledge about quality and production of berries to benefit health of the consumers and maintain profitable production using sustainable systems. The objective will be achieved by focusing on four main areas of research and development: From genetics to variety evaluation Nursery production systems and plant quality control Plant physiology, biochemistry and molecular biology, as well as cultural management Health for the consumer: components and factors affecting berries'' nutritional value Specifically, the journal will cover berries (strawberry, raspberry, blackberry, blueberry, cranberry currants, etc.), as well as grapes and small soft fruit in general (e.g., kiwi fruit). It will publish research results covering all areas of plant breeding, including plant genetics, genomics, functional genomics, proteomics and metabolomics, plant physiology, plant pathology and plant development, as well as results dealing with the chemistry and biochemistry of bioactive compounds contained in such fruits and their possible role in human health. Contributions detailing possible pharmacological, medical or therapeutic use or dietary significance will be welcomed in addition to studies regarding biosafety issues of genetically modified plants.