Using cell monolayer rheology to probe average single cell mechanical properties.

IF 1 4区医学 Q4 BIOPHYSICS

Biorheology Pub Date : 2015-01-01 DOI:10.3233/BIR-15070

M. Sander, J. Flesch, A. Ott

引用次数: 6

Abstract

The cell monolayer rheology technique consists of a commercial rotational rheometer that probes the mechanical properties of a monolayer of isolated cells. So far we have described properties of an entire monolayer. In this short communication, we show that we can deduce average single cell properties. Results are in very good agreement with earlier work on single cell mechanics. Our approach provides a mean of 105-106 adherent cells within a single experiment. This makes the results very reproducible. We extend our work on cell adhesion strength and deduce cell adhesion forces of fibroblast cells on fibronectin coated glass substrates.

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利用细胞单层流变学研究平均单细胞力学性能。

细胞单层流变学技术由一个商业旋转流变仪组成，该流变仪探测分离细胞单层的机械特性。到目前为止，我们已经描述了整个单层的性质。在这个简短的交流中，我们展示了我们可以推断出平均单细胞的性质。结果与早期的单细胞力学工作非常吻合。我们的方法在单个实验中平均提供105-106个贴壁细胞。这使得结果非常具有可重复性。我们扩展了细胞粘附强度的研究，并推导了成纤维细胞在纤维连接蛋白涂层玻璃基板上的粘附力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biorheology 医学-工程：生物医学

CiteScore

2.00

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Biorheology is an international interdisciplinary journal that publishes research on the deformation and flow properties of biological systems or materials. It is the aim of the editors and publishers of Biorheology to bring together contributions from those working in various fields of biorheological research from all over the world. A diverse editorial board with broad international representation provides guidance and expertise in wide-ranging applications of rheological methods to biological systems and materials. The scope of papers solicited by Biorheology extends to systems at different levels of organization that have never been studied before, or, if studied previously, have either never been analyzed in terms of their rheological properties or have not been studied from the point of view of the rheological matching between their structural and functional properties. This biorheological approach applies in particular to molecular studies where changes of physical properties and conformation are investigated without reference to how the process actually takes place, how the forces generated are matched to the properties of the structures and environment concerned, proper time scales, or what structures or strength of structures are required.