Serologic screening for 13 infectious agents in roe deer (Capreolus capreolus) in Flanders

Q1 Environmental Science
P. Tavernier, S. Sys, K. De Clercq, I. de Leeuw, A. Caij, Miet I De Baere, N. De Regge, D. Fretin, V. Roupie, M. Govaerts, P. Heyman, D. Vanrompay, L. Yin, I. Kalmar, V. Suin, B. Brochier, A. Dobly, S. De Craeye, Sophie Roelandt, E. Goossens, S. Roels
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引用次数: 36

Abstract

Introduction In order to investigate the role of roe deer in the maintenance and transmission of infectious animal and human diseases in Flanders, we conducted a serologic screening in 12 hunting areas. Materials and methods Roe deer sera collected between 2008 and 2013 (n=190) were examined for antibodies against 13 infectious agents, using indirect enzyme-linked immunosorbent assay, virus neutralisation, immunofluorescence, or microagglutination test, depending on the agent. Results and discussion High numbers of seropositives were found for Anaplasma phagocytophilum (45.8%), Toxoplasma gondii (43.2%) and Schmallenberg virus (27.9%), the latter with a distinct temporal distribution pattern following the outbreak in domestic ruminants. Lower antibody prevalence was found for Chlamydia abortus (6.7%), tick-borne encephalitis virus (5.1%), Neospora caninum (4.8%), and Mycobacterium avium subsp paratuberculosis (4.1%). The lowest prevalences were found for Leptospira (1.7%), bovine viral diarrhoea virus 1 (1.3%), and Coxiella burnetii (1.2%). No antibodies were found against Brucella sp., bovine herpesvirus 1, and bluetongue virus. A significant difference in seroprevalence between ages (higher in adults >1 year) was found for N. caninum. Four doubtful reacting sera accounted for a significant difference in seroprevalence between sexes for C. abortus (higher in females). Conclusions Despite the more intensive landscape use in Flanders, the results are consistent with other European studies. Apart from maintaining C. abortus and MAP, roe deer do not seem to play an important role in the epidemiology of the examined zoonotic and domestic animal pathogens. Nevertheless, their meaning as sentinels should not be neglected in the absence of other wild cervid species.
佛兰德斯鹿(Capreolus Capreolus) 13种感染因子的血清学筛查
为了调查狍在佛兰德斯地区动物和人类传染病维持和传播中的作用,我们在12个狩猎区进行了血清学筛查。材料和方法采用间接酶联免疫吸附试验、病毒中和试验、免疫荧光试验或微凝集试验,对2008年至2013年收集的獐血清(n=190)进行13种传染性病原体的抗体检测。结果与讨论嗜吞噬细胞无形体(45.8%)、刚地弓形虫(43.2%)和施马伦贝格病毒(27.9%)血清阳性,施马伦贝格病毒在家畜反刍动物中有明显的时间分布规律。流产衣原体(6.7%)、蜱传脑炎病毒(5.1%)、犬新孢子虫(4.8%)和鸟分枝杆菌副结核次(4.1%)的抗体患病率较低。钩端螺旋体(1.7%)、牛病毒性腹泻病毒1型(1.3%)和伯纳蒂克希菌(1.2%)的患病率最低。未发现布鲁氏菌、牛疱疹病毒1型和蓝舌病病毒的抗体。在不同年龄的人群中,犬嗜血杆菌的血清流行率有显著差异(在10 - 10岁的成年人中较高)。四种可疑反应血清在两性之间的血清阳性率有显著差异(女性较高)。尽管佛兰德斯的景观利用更为密集,但结果与欧洲其他研究结果一致。除了维持C. abortus和MAP外,狍在所检查的人畜共患和家畜病原体的流行病学中似乎没有发挥重要作用。然而,在缺乏其他野生鹿科物种的情况下,它们作为哨兵的意义不应被忽视。
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来源期刊
Infection Ecology and Epidemiology
Infection Ecology and Epidemiology Environmental Science-Environmental Science (miscellaneous)
CiteScore
8.70
自引率
0.00%
发文量
4
审稿时长
12 weeks
期刊介绍: Infection Ecology & Epidemiology aims to stimulate inter-disciplinary collaborations dealing with a range of subjects, from the plethora of zoonotic infections in humans, over diseases with implication in wildlife ecology, to advanced virology and bacteriology. The journal specifically welcomes papers from studies where researchers from multiple medical and ecological disciplines are collaborating so as to increase our knowledge of the emergence, spread and effect of new and re-emerged infectious diseases in humans, domestic animals and wildlife. Main areas of interest include, but are not limited to: 1.Zoonotic microbioorganisms 2.Vector borne infections 3.Gastrointestinal pathogens 4.Antimicrobial resistance 5.Zoonotic microbioorganisms in changing environment
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