An Experimental Study on Intestine Transmission Function in SD Rats With Slow Transit Constipation After Electro-acupuncture Treatment in BL32 Acupoints

IF 0.2 4区 医学 Q4 INTEGRATIVE & COMPLEMENTARY MEDICINE
Muwen Qu, Lingling Yuan, Fei Jia, Jun-Yi Li
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Abstract

Objective:This study aims to use loperamide by gavage in SD rats to establish the model of STC, and evaluate the degree of similarity between the model and clinical diseases. Furthermore, the effect of electro-acupuncture when stimulating BL32 acupoints in SD rats with slow transit constipation were observed to determine changes in intestinal transmission function. Methods:Thirty SD rats were selected and divided randomly into three groups. Rats in the model group were given 2 mg/kg/d of loperamide tablets by gavage for 15 days. The morphology of the feces of rats was observed, and the dry weight of the feces and the time of first black feces movement with black ink by gavage after molding were measured. After 20 days of electro-acupuncture treatment at the BL32 acupoint, the time of first black feces movement, the ratio of black ink length of the entire length of the intestine, and contraction amplitude and frequency of contraction were measured. Results: The time of black feces movement in the two model groups was significantly prolonged (P<0.05).Furthermore, fecal grains, dry weight and moisture content were significantly reduced (P<0.05). On the 10thand 20thday of treatment, the time of black feces movementin the treatment group significantly decreased (P<0.05). The ratio of black ink length of the entire length of the intestine in the electro-acupuncture group was significantly higher than that in the model group (P<0.05). Moreover, contraction amplitude was significantly higher than in the model control group (P<0.05). Conclusion: Through loperamide gavage, the model of slow transit constipation in SD rats was successfully established in a relatively short period of time. Electro-acupuncture treatment had a rapid onset of action, and its mechanism might have been caused through strengthening the ability of intestinal smooth muscle contraction, and are independent with increased smooth muscle contraction frequency.
电针BL32穴治疗慢传输型便秘SD大鼠肠传功能的实验研究
目的:本研究旨在用洛哌丁胺灌胃SD大鼠建立STC模型,并评价模型与临床疾病的相似程度。进一步观察电针刺激慢传输型便秘SD大鼠BL32穴位对肠传递功能的影响。方法:选取SD大鼠30只,随机分为3组。模型组大鼠给予洛哌丁胺片2 mg/kg/d灌胃,连续15 d。观察大鼠粪便形态,测定大鼠成型后粪便干重及灌胃后第一次以黑色墨水移动黑色粪便的时间。电针治疗BL32穴20 d后,测量大鼠第一次移动黑色粪便的时间、黑色墨水长度与整个肠长之比、收缩幅度和收缩频率。结果:两模型组大鼠黑色粪便移动时间均显著延长(P<0.05)。粪便颗粒、干重和水分含量显著降低(P<0.05)。在治疗第10和20天,治疗组黑色粪便移动时间显著缩短(P<0.05)。电针组大鼠黑墨长度占全肠长度之比显著高于模型组(P<0.05)。收缩幅度明显高于模型对照组(P<0.05)。结论:通过洛哌丁胺灌胃,在较短时间内成功建立SD大鼠慢传输型便秘模型。电针治疗起效快,其作用机制可能是通过增强肠道平滑肌收缩能力引起的,且与平滑肌收缩频率的增加是独立的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Acupuncture & Electro-Therapeutics Research
Acupuncture & Electro-Therapeutics Research 医学-全科医学与补充医学
CiteScore
0.50
自引率
66.70%
发文量
9
审稿时长
>12 weeks
期刊介绍: The aim of the journal is to provide an international forum for the exchange of ideas and promotion of basic and clinical research in acupuncture, electro-therapeutics, and related fields. The journal was established in order to make acupuncture and electro-therapeutics a universally acceptable branch of medicine through multidisciplinary research based on scientific disciplines. The final goal is to provide a better understanding of both the beneficial and adverse effects of these treatments in order to supplement or improve existing methods of diagnosis, prognosis, treatment, and prevention of diseases in both Western and Oriental medicine.
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