Hepatoprotective Impact of Vitamin C against Mitochondrial Dysfunction Induced by Malathion on Rat Liver

Current science international Pub Date : 2022-01-01 DOI:10.36632/csi/2022.11.1.1

M. Hamed, S. Nasr, Sanaa M. Abdulrahman

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Abstract

The present study is designed to investigate the possible hepatoprotective effect of vitamin C on liver of rats. A total number of 32 female albino rats. Animals were divided into four groups; Group 1: Negative control group Group 2: Positive control group, animals in this group were administrated with Malathion (50 mg/kg/day) intraperitoneally, Group 3: Animals in this group were orally administrated with vitamin C (100 mg/kg/day) and Group 4: Animals in this group were administrated with malathion (50 mg/kg/day) intraperitoneally with vitamin C (100 mg/kg/day) orally. The blood samples were centrifuged in cooling centrifuge to separate serum to measure aspartate amino transferase (AST) and alanine amino transferase (ALT) activity, total protein and albumin. Tissues of the liver was homogenized to estimate the content of reduced glutathione (GSH), malondialdehyde (MDA) and the activity of copper-zinc superoxide dismutase (SOD). Also another portion of the liver tissue was homogenized to isolate mitochondria to estimate the content of GSH, MDA and the activity of SOD, nuclear factor-erythroid 2-related factor2 (Nrf2) and kelch like ECH associated protein 1(keap1). Histological examination of the tissues was conducted after removal of liver tissues from rats. The results indicated a significant increase MDA level in the liver and mitochondria homogenates of malathion-treated rats compared to the negative control ones. Malathion also resulted in a significant decrease in liver and mitochondrial GSH content. ALT & AST activities significantly increased while protein and albumin significantly decreased compared to the negative control. The obtained results illustrated that rats treated with Malathion for 30 days showed a significant decrease in Nrf2 while Keap1 decreased significantly compared to the negative control group. Animals treated with malathion+ vitamin C showed an improvement in all these parameters and the histological study confirmed these result. In a conclusion, vitamin C can ameliorate the hazardous effects of Malathion.

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维生素C对马拉硫磷所致大鼠肝脏线粒体功能障碍的保护作用

本研究旨在探讨维生素C对大鼠肝脏可能的保肝作用。共32只雌性白化大鼠。动物被分成四组;组1:阴性对照组组2:阳性对照组，该组动物腹腔注射马拉硫磷(50 mg/kg/d)，组3:该组动物口服维生素C (100 mg/kg/d)，组4:该组动物腹腔注射马拉硫磷(50 mg/kg/d)并口服维生素C (100 mg/kg/d)。采用冷却离心分离法分离血清，测定天冬氨酸氨基转移酶(AST)和丙氨酸氨基转移酶(ALT)活性、总蛋白和白蛋白。肝脏组织匀浆，测定还原性谷胱甘肽(GSH)、丙二醛(MDA)含量和铜锌超氧化物歧化酶(SOD)活性。另取肝组织匀浆分离线粒体，测定GSH、MDA含量及SOD、核因子-红细胞2相关因子2 (Nrf2)、kelch样ECH相关蛋白1(keap1)活性。取大鼠肝组织后进行组织组织学检查。结果表明，与阴性对照组相比，马拉硫磷处理大鼠肝脏和线粒体匀浆中MDA水平显著升高。马拉硫磷还导致肝脏和线粒体谷胱甘肽含量显著降低。与阴性对照组相比，ALT和AST活性显著升高，蛋白和白蛋白活性显著降低。结果显示，与阴性对照组相比，马拉硫磷治疗30天大鼠Nrf2显著降低，Keap1显著降低。用马拉硫磷+维生素C治疗动物，这些指标均有改善，组织学研究证实了这一结果。总之，维生素C可以减轻马拉硫磷的危害。

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Current science international

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