Baicalein Suppressed Cervical Cancer Tumourogenesis by Modulating circ_0007364/microRNA-665/Tumor Protein D52 Axis

IF 0.4 4区 医学 Q4 PHARMACOLOGY & PHARMACY
X. Liu, J-J Xie, Jingfang Zhu
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引用次数: 0

Abstract

Baicalein has anti-tumor effect and participates in multi-channel regulation mechanism. However, the mechanism of baicalein action has not been fully clarified in cervical cancer. Tumor protein D52, circ_0007364 and microRNA-665 expression were determined by quantitative reverse transcription-polymerase chain reaction. Tumor protein D52 and apoptosis-related proteins were detected by Western blot. Cell proliferation was measured via methyl thiazolyl tetrazolium, 5-ethynyl-2'-deoxyuridine and colony formation assays. The associations among microRNA-655, circ_0007364 and tumor protein D52 were determined by dual luciferase report assay. Cell invasion and apoptosis were assessed by Transwell assay and flow cytometry. The function experiments were carried out in vivo and in vitro . Baicalein significantly decreased circ_0007364 and tumor protein D52 expression and increased microRNA-665 expression in cervical cancer cell samples. Circ_0007364 or tumor protein D52 introduction and microRNA-665 down regulation weakened the suppressive effects of baicalein in cervical cancer cells. Moreover, circ_0007364 functioned by binding to microRNA-665 to regulate tumor protein D52 and affect cellular process in cervical cancer. Our study showed that baicalein inhibited cervical cancer cell growth via regulating circ_0007364/microRNA-665/tumor protein D52 axis, further understanding the anticancer mechanism of baicalein and contributing to find more effective anticancer drug components for cervical cancer.
黄芩素通过调节circ_0007364/microRNA-665/肿瘤蛋白D52轴抑制宫颈癌的发生
黄芩素具有抗肿瘤作用,参与多通道调控机制。然而,黄芩素在宫颈癌中的作用机制尚未完全阐明。采用定量逆转录-聚合酶链反应检测肿瘤蛋白D52、circ_0007364和microRNA-665的表达。Western blot检测肿瘤蛋白D52及凋亡相关蛋白的表达。通过甲基噻唑四氮唑、5-乙基-2'-脱氧尿苷和集落形成试验检测细胞增殖。采用双荧光素酶报告法检测microRNA-655、circ_0007364与肿瘤蛋白D52的相关性。采用Transwell法和流式细胞术观察细胞侵袭和凋亡情况。在体内和体外进行了功能实验。黄芩素显著降低宫颈癌细胞样品中circ_0007364和肿瘤蛋白D52的表达,升高microRNA-665的表达。Circ_0007364或肿瘤蛋白D52的引入和microRNA-665的下调削弱了黄芩素对宫颈癌细胞的抑制作用。此外,circ_0007364通过与microRNA-665结合,调控肿瘤蛋白D52,影响宫颈癌的细胞过程。本研究发现黄芩素通过调控circ_0007364/microRNA-665/肿瘤蛋白D52轴抑制宫颈癌细胞生长,进一步了解黄芩素的抗癌机制,有助于发现更有效的宫颈癌抗癌药物成分。
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来源期刊
自引率
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审稿时长
2 months
期刊介绍: The Indian Journal of Pharmaceutical Sciences (IJPS) is a bi-monthly Journal, which publishes original research work that contributes significantly to further the scientific knowledge in Pharmaceutical Sciences (Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy and Phytochemistry, Pharmacology and Therapeutics, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Pharmacovigilance, Pharmacoepidemiology, Pharmacoeconomics, Drug Information, Patient Counselling, Adverse Drug Reactions Monitoring, Medication Errors, Medication Optimization, Medication Therapy Management, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest). The Journal publishes original research work either as a Full Research Paper or as a Short Communication. Review Articles on current topics in Pharmaceutical Sciences are also considered for publication by the Journal.
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