Bioassay Guided Isolation of Anti-Inflammatory Compounds from Bauhinia variegata L.: A Key Ingredient in Herbo-Mineral Formulation, Gandmala Kandan Ras

IF 0.4 4区 医学 Q4 PHARMACOLOGY & PHARMACY
V. B. Tatipamula, K. Killari, Nguyen Huy Thuan, D. Prasanth, S. Panda, P. K. Pasala, Alekhya Ketha
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Abstract

: Anti-inflammatory compounds from B. variegata L. Medicinal herb Bauhinia variegata L. is the main ingredient in “Gandmala Kandan Ras” (GKR) herbo-mineral medicine used in Ayurveda for the treatment of swelling, inflammation and tumors. This study aimed to isolate the anti-inflammatory compounds from the methanolic extract of aerial parts of Bauhinia variegata . Through bioassay-guided isolation, three known flavonoids, namely kaempferol, ombuin and quercetin were identified from methanolic extract of aerial parts of Bauhinia variega . The primary screening by protein denaturation method revealed a significant percentage of inhibition of protein denaturation of compounds kaempferol and quercetin. The anti-inflammatory assays against COX-1/2 enzymes showed significant anti-inflammatory activity of these two compounds, compared to the standard drug, indomethacin. Of which, quercetin as a potent non-selective inhibitor of COX1 and COX2 with IC 50 values of 172.05±4.29 and 220.62±9.13 nM, respectively; while kaempferol showed selective COX2 inhibition with the IC 50 of 154.86±5.60 nM. The results provided evidence that supports the Ayurvedic usage of Gandmala Kandan Ras formulation in the treatment of inflammation, which was attributed to the natural active kaempferol and quercetin. The results indicated that plant Bauhinia variegata could be considered as an excellent natural source of remedial medicine for inflammation.
生物测定法指导下从紫荆花中分离抗炎化合物——一种草药矿物制剂中的关键成分
药用植物紫荆(bahinia variegata L.)是印度草药中用于治疗肿胀、炎症和肿瘤的“Gandmala Kandan Ras”(GKR)草药矿物药物的主要成分。本研究旨在从紫荆地上部甲醇提取物中分离抗炎化合物。通过生物测定引导分离,从紫荆花地上部甲醇提取物中分离到山奈酚、甘露素和槲皮素3种已知的黄酮类化合物。通过蛋白变性法初步筛选发现山奈酚和槲皮素对蛋白变性有显著的抑制作用。对COX-1/2酶的抗炎实验表明,与标准药物吲哚美辛相比,这两种化合物具有显著的抗炎活性。其中槲皮素为COX1和COX2的有效非选择性抑制剂,ic50值分别为172.05±4.29和220.62±9.13 nM;山奈酚对COX2有选择性抑制作用,ic50为154.86±5.60 nM。结果提供了证据,支持阿育吠陀使用甘马拉Kandan Ras制剂治疗炎症,这是由于天然活性山奈酚和槲皮素。结果表明,紫荆花是一种很好的治疗炎症的天然药物。
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审稿时长
2 months
期刊介绍: The Indian Journal of Pharmaceutical Sciences (IJPS) is a bi-monthly Journal, which publishes original research work that contributes significantly to further the scientific knowledge in Pharmaceutical Sciences (Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy and Phytochemistry, Pharmacology and Therapeutics, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Pharmacovigilance, Pharmacoepidemiology, Pharmacoeconomics, Drug Information, Patient Counselling, Adverse Drug Reactions Monitoring, Medication Errors, Medication Optimization, Medication Therapy Management, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest). The Journal publishes original research work either as a Full Research Paper or as a Short Communication. Review Articles on current topics in Pharmaceutical Sciences are also considered for publication by the Journal.
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