Zaleya decandra exerts anti-cancer activity in HCT-116 cancer cells by impairing mitochondrial activity

IF 0.9 4区医学 Q4 PHARMACOLOGY & PHARMACY

Bangladesh Journal of Pharmacology Pub Date : 2019-01-01 DOI:10.3329/bjp.v14i4.42098

M. Deivasigamani, A. Chandrasekar, Sridhar Muthusami, Gopalakrishnan Velliyur Kanniappan, Hariprasath Lakshmanan

{"title":"Zaleya decandra exerts anti-cancer activity in HCT-116 cancer cells by impairing mitochondrial activity","authors":"M. Deivasigamani, A. Chandrasekar, Sridhar Muthusami, Gopalakrishnan Velliyur Kanniappan, Hariprasath Lakshmanan","doi":"10.3329/bjp.v14i4.42098","DOIUrl":null,"url":null,"abstract":"An attempt has been made to investigate the role of Zaleya decandra on the survival of HCT colon cancer cells in vitro. The cell viability and apoptosis were assessed using MTT assay and acridine orange/ethidium bromide staining, respectively. Mitrochondrial membrane permeability was assessed using rhodamine-123 staining. The activities of caspases were evaluated using ELISA kits. Treatment with Z. decandra extract increased the production of ROS in HCT cells and impaired the mitochondrial activity as evidenced by decreased reduction of tetrazolium salt and enhanced mitochondrial membrane permeability. The onset of apoptosis was evident in cells treated with the extact in acridine orange/ethidium bromide staining and was associated with elevated levels of initiator and effector caspases in the conditioned medium. In conclusion, Z. decandra treatment promoted the apoptosis of HCT colon cancer cells. Video Clip of Methodology: 6 min 53 sec: Full Screen Alternate","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":null,"pages":null},"PeriodicalIF":0.9000,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/bjp.v14i4.42098","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bangladesh Journal of Pharmacology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3329/bjp.v14i4.42098","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}

引用次数: 2

Abstract

An attempt has been made to investigate the role of Zaleya decandra on the survival of HCT colon cancer cells in vitro. The cell viability and apoptosis were assessed using MTT assay and acridine orange/ethidium bromide staining, respectively. Mitrochondrial membrane permeability was assessed using rhodamine-123 staining. The activities of caspases were evaluated using ELISA kits. Treatment with Z. decandra extract increased the production of ROS in HCT cells and impaired the mitochondrial activity as evidenced by decreased reduction of tetrazolium salt and enhanced mitochondrial membrane permeability. The onset of apoptosis was evident in cells treated with the extact in acridine orange/ethidium bromide staining and was associated with elevated levels of initiator and effector caspases in the conditioned medium. In conclusion, Z. decandra treatment promoted the apoptosis of HCT colon cancer cells. Video Clip of Methodology: 6 min 53 sec: Full Screen Alternate

查看原文本刊更多论文

扎莱菊通过损害线粒体活性对HCT-116癌细胞发挥抗癌作用

我们试图研究扎莱亚对体外HCT结肠癌细胞存活的作用。采用MTT法和吖啶橙/溴化乙锭染色法检测细胞活力和凋亡情况。罗丹明-123染色法测定线粒体膜通透性。采用酶联免疫吸附试验(ELISA)检测半胱天冬酶活性。枸杞提取物增加了HCT细胞中ROS的产生，并损害了线粒体活性，四氮唑盐的减少和线粒体膜通透性的增强证明了这一点。在吖啶橙/溴化乙啶染色中，用提取物处理的细胞明显发生凋亡，并且与条件培养基中引发剂和效应剂半胱天冬酶水平升高有关。综上所述，枸杞对HCT结肠癌细胞凋亡有促进作用。方法论视频剪辑:6分53秒:全屏交替播放

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Bangladesh Journal of Pharmacology PHARMACOLOGY & PHARMACY-

CiteScore

1.50

自引率

18.80%

发文量

审稿时长

>12 weeks

期刊介绍： Bangladesh Journal of Pharmacology (Bangladesh J Pharmacol) is an open access, video component, peer-reviewed journal of the Bangladesh Pharmacological Society (BDPS). A scholarly publication is defined ''open access'' when there are no financial, legal or technical barriers to accessing it. Anyone can read, download, copy, distribute, print or use it in educational purpose within the legal agreements. Peer review of a manuscript means the evaluation of research work by one or more people with similar competences. It can be open or blind. We do the single-blind peer review. Our readers want to know the methodology used by the researchers which is very important to get the results. The video clip of one of the methodologies is prepared by the authors and submit it to publish.