Biocontrol of Foot and Root Rot Disease of Grasspea (Lathyrus sativus) by Dual Inoculation with Rhizobium and Arbuscular Mycorrhiza

Bangladesh journal of microbiology Pub Date : 2019-01-01 DOI:10.3329/bjm.v34i2.39622

Mushfika Rahman, Me Ali, F. Alam, M. Banu, M. Faruk, M. Bhuiyan

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引用次数: 2

Abstract

The present study was carried out to evaluate the effect of indigenous arbuscular mycorrhizal fungi (AMF) and Rhizobium (R) on plant growth and their biocontrol against grasspea foot and root rot disease caused by Sclerotium rolfsii. The bio-control potential of these bio-agents against foot and root rot pathogen was carried out under pot culture condition using AMF alone or in combination with rhizobial inoculum in the nethouse of Soil Science Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur during 2014-2015 through 2015-2016. The experiment was designed in RCBD with 8 treatments and 4 replications. Grasspea variety BARI Khesari-1 was used as a test crop. Peat based rhizobial inoculum (BARI RLs-10) was used in this experiment @ 50 g kg-1 seed. The AM fungi used in this experiment were Glomus fusianum, Glomus macrocarpum, Glomus warcuppi, Acaulospora foveata, Acaulospora denticulate, Gigaspora albida, Gigaspora rosea, Glomus spp. etc. Soil based AM inoculum containing about approximate 252 spores and infected root pieces of the host plant was used pot-1. There were eight treatments viz. T1: Arbuscular mycorrhiza (AM), T2: Rhizobium, T3: AM + Rhizobium, T4: Sclerotium rolfsii, T5: Sclerotium rolfsii + AM, T6: Sclerotium rolfsii + Rhizobium, T7: Sclerotium rolfsii + AM + Rhizobium and T8: Control. Dual inoculation (AM + Rhizobium) increased 20-25% germination, 50-100% seed yield and 36-98% stover yield compared to control. Dual inoculation reduced 44-48% foot and root rot disease compared to control. On the contrary, Sclerotium rolfsii + Rhizobium, Sclerotium rolfsii + AM, and Sclerotium rolfsii + AM + Rhizobium reduced 12-17%, 16-20% and 28-31% foot and root rot disease, respectively compared to only Sclerotium rolfsii trearment. Therefore, arbuscular mycorrhizal fungal species and its combination with rhizobial inoculum were significant both in the formation and effectiveness of AM symbiosis and the reduction of foot and root rot incidence in grasspea plants. Use of these bio-control agents could be promoted as an active component of bio-intensive Integrated Disease Management Program (IDMP) under organic mode. Bangladesh J Microbiol, Volume 34 Number 2 December 2017, pp 109-117

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根瘤菌和丛枝菌根双接种防治草地足腐病和根腐病

本试验研究了土生丛枝菌根真菌(AMF)和根瘤菌(R)对植物生长的影响及其对草足病和根腐病的防效。2014-2015年至2015-2016年，在加济浦尔Joydebpur孟加拉国农业研究所土壤科学处的网棚中，在盆栽条件下，单独使用AMF或与根瘤菌联合接种，研究了这些生物制剂对足腐病和根腐病的生物防治潜力。试验采用RCBD设计，8个处理，4个重复。以牧草品种BARI Khesari-1为试验作物。本试验采用以泥炭为基础的根瘤菌接种剂(BARI RLs-10)，种子质量为50 g kg-1。本实验使用的AM真菌有Glomus fususianum、Glomus macrocarpum、Glomus warcuppi、Acaulospora foveata、Acaulospora dendenulate、Gigaspora albida、Gigaspora rosea、Glomus spp等。盆栽1采用含约252个孢子和侵染的寄主根片的土壤AM接种物。8个处理分别是:T1:丛枝菌根(AM)、T2:根瘤菌、T3: AM +根瘤菌、T4:罗尔夫菌核菌、T5:罗尔夫菌核菌+ AM、T6:罗尔夫菌核菌+根瘤菌、T7:罗尔夫菌核菌+ AM +根瘤菌和T8:对照。与对照相比，双接种(AM +根瘤菌)发芽率提高20-25%，种子产量提高50-100%，秸秆产量提高36-98%。与对照相比，双重接种减少了44-48%的足腐病和根腐病。与单纯菌核处理相比，菌核+根瘤菌、菌核+ AM和菌核+ AM +根瘤菌分别减少了12-17%、16-20%和28-31%的根腐病和足腐病。因此，丛枝菌根真菌种类及其与根瘤菌接种量的组合在草种植物AM共生的形成和有效性以及减少足腐病和根腐病发生率方面都具有重要意义。在有机模式下，这些生物防治剂可作为生物密集型疾病综合管理计划(IDMP)的有效组成部分加以推广。孟加拉微生物学杂志，2017年12月第34卷第2期，109-117页

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Bangladesh journal of microbiology

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