GREEN SYNTHESIS OF SILVER NANOPARTICLES FROM Scoparia dulcis L. PLANT EXTRACT AND ITS In-vitro ACETYLCHOLINESTERASE, ANTIOXIDANT ACTIVITY

Abstract

Silver oxide nanoparticles exhibit distinctive material and biotic properties that contain passionate research impact as vital applications in the pharmaceutical, biomedical, paper and textile industries. The goal of the current task is to synthesize AgNPs through a facile, low-cost, environmentally friendly route. In this method, we use the aqueous leaf extract of Scoparia dulcis Linn, found in the Western Ghats region, because the use of an aqueous medium is critical for reducing time and minimizing the possibility of side effects. The synthesized Ag nanoparticles were evaluated using UV-Visible, FTIR technique, scanning electron microscopy (SEM) with EADX, transmission electron microscopy (TEM), X-ray powder diffraction (XRD). Results confirmed that UV-visible spectroscopic analysis showed the absorbance peak at 460 nm, which implies the formation of silver nanoparticles. The FTIR spectrum aimed to such that biomolecules involved in wrapping and binding AgNPs synthesized by the plant extract. SEM and TEM are used to visualize the size and shape of nanoparticles. The XRD analysis revealed spherical structures with an average grain size of 21nm and a diameter range of 2 to 20nm. The EDAX study also exhibits the presence of 86.34 % Silver and 13.6 % Oxygen. Alzheimer's disease symptoms were controlled with a drug known as an acetylcholinesterase inhibitor. The in vitro acetylcholinesterase inhibitory activity of the AgNPs from Scoparia dulcis plant extract was evaluated using an Ellman's test with an IC50 value of 149µg/ml. The generated nanoparticles significantly inhibit AChE. The SD-AgNPs were examined for antioxidant activity via the DPPH and ABTS assays having IC50 values of 73.29µg/ml and 55.29µg/ml, respectively