BIOANALYTICAL METHOD PROCESS OF CHROMATOGRAPHIC ANALYSIS OF TIZANIDINE IN THE FORMULATION AND HUMAN PLASMA

IF 0.5 Q4 EDUCATION & EDUCATIONAL RESEARCH

Rasayan Journal of Chemistry Pub Date : 2023-01-01 DOI:10.31788/rjc.2023.1618127

引用次数: 0

Abstract

An optimized, simple, accurate method is developed to quantify Tizanidine in bulk and pharmaceutical formulation using the RP – HPLC method using a silica ODS C18 column with the aid of MeOH: H2O:0.01M orthophosphoric acid ( 95:4:1 v/v/v) as a mobile phase and deduced at 320nm. The RT value was found to be 2.274 with a resolution of 1.167 respectively. The spiking studies of Tizanidine in human plasma were also carried out. The linearity studies of TZA (10 -50 mcg/mL) were found to be R2 =0.99979 (API) and R2 = 0.8155 respectively. The suggested study was validated as per the criteria of the ICH guidelines. The proposed study could be easily adopted in the pharmaceutical industry for performing quantitative analyses

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替扎尼定制剂及人血浆色谱分析的生物分析方法

以二氧化硅ODS C18为色谱柱，MeOH: H2O:0.01M正磷酸(95:4:1 v/v/v)为流动相，在320nm处建立了一种简便、准确的定量替扎尼定原料药和制剂的RP - HPLC方法。RT值为2.274，分辨率为1.167。同时进行了替扎尼定在人血浆中的峰值研究。TZA (10 ~ 50 mcg/mL)的线性关系R2 =0.99979 (API)， R2 = 0.8155。建议的研究按照ICH指南的标准进行了验证。所提出的研究可以很容易地应用于制药行业进行定量分析

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来源期刊

Rasayan Journal of Chemistry Energy-Energy (all)

CiteScore

1.90

自引率

0.00%

发文量

196

期刊介绍： RASĀYAN Journal of Chemistry [RJC] signifies a confluence of diverse streams of chemistry to stir up the cerebral powers of its contributors and readers. By introducing the journal by this name, we humbly intent to provide an open platform to all researchers, academicians and readers to showcase their ideas and research findings among the people of their own fraternity and to share their vast repository of knowledge and information. The journal seeks to embody the spirit of enquiry and innovation to augment the richness of existing chemistry literature and theories. We also aim towards making this journal an unparalleled reservoir of information and in process aspire to inculcate and expand the research aptitude.