Active Hexose Correlated Compound Activates Immune Function to Decrease Chlamydia trachomatis Shedding in a Murine Stress Model

T. Belay, Chih-lung Fu, Anthony Woart
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引用次数: 7

Abstract

A cold-induced stress mouse model for investigating chlamydia genital infection and immune response analysis was established in our laboratory. Previous results showed that cold-induced stress results in suppression of the immune response and increased intensity of chlamydia genital infection in the mouse model. The purpose of the present study was to evaluate the potential therapeutic value of active hexose correlated compound (AHCC) against chlamydia genital infection in mice. AHCC is an extract of mushroom commonly used as a dietary supplement is known to boost the immune system. Mice were infected intravaginally with Chlamydia trachomatis after a 24-day cold-stress application. Oral administration of AHCC to stressed or non-stressed mice was carried out seven days before infection and during the course of infection along with cervicovaginal swabbing. Cytokine production by peritoneal and splenic T cells isolated from AHCC-fed stressed mice and non-stressed mice was measured ELISA. Splenic T cells from both animal groups were co-cultured with mouse monocyte J774.2 cell line or cultured by addition of supernatants of AHCC-treated J774.2 cell line for 24 hours. Infection studies showed that AHCC-feeding compared to phosphate buffered saline (PBS)-feeding to stressed mice resulted in reduced Chlamydia trachomatis shedding from the genital tract. Levels of tumor necrosis factor-alpha (TNF-α) and interleukin 6 (IL-6) were significantly increased in stressed mice receiving AHCC compared to stressed mice receiving PBS. Production of interferon gamma (IFN-γ) and interleukin 2 (IL-2) in the AHCC group was significantly high compared to production in PBS-fed group. Splenic T cells from stressed and non-stressed cultured with supernatants of AHCC-treated J774.2 cell line resulted in significantly increased TNF-α or IFN-γ production. Results obtained in this study show that AHCC improves the function of immune cells as indicated by the restoration of levels of cytokines production that were suppressed under cold induced-stress conditions. This is the first report showing that oral administration of AHCC enhances the function of the immune system, which could result in increased resistance of the host to chlamydia genital infection.
活性己糖相关化合物激活免疫功能减少小鼠应激模型中沙眼衣原体脱落
本实验室建立了用于研究衣原体生殖感染和免疫应答分析的冷应激小鼠模型。先前的研究结果表明,在小鼠模型中,冷诱导的应激导致免疫应答抑制和衣原体生殖器感染强度增加。本研究的目的是评价活性己糖相关化合物(AHCC)对小鼠衣原体生殖器感染的潜在治疗价值。AHCC是一种蘑菇提取物,通常用作膳食补充剂,可以增强免疫系统。小鼠经阴道感染沙眼衣原体24天后进行冷应激。在感染前7天和感染过程中,对应激或非应激小鼠口服AHCC,并擦拭宫颈阴道。ELISA法测定ahcc饲喂应激小鼠和非应激小鼠腹膜和脾T细胞产生细胞因子的情况。两组小鼠脾T细胞分别与小鼠单核细胞J774.2细胞系共培养或加入ahcc处理过的J774.2细胞系上清液培养24h。感染研究表明,与磷酸缓冲盐水(PBS)喂养相比,ahcc喂养应激小鼠导致沙眼衣原体从生殖道脱落减少。与接受PBS的应激小鼠相比,接受AHCC的应激小鼠的肿瘤坏死因子-α (TNF-α)和白细胞介素6 (IL-6)水平显著升高。与pbs喂养组相比,AHCC组的干扰素γ (IFN-γ)和白细胞介素2 (IL-2)的产生显著增加。用ahcc处理的J774.2细胞株上清液培养应激和非应激的脾T细胞,TNF-α或IFN-γ的产生显著增加。本研究的结果表明,AHCC可以改善免疫细胞的功能,恢复在冷诱导应激条件下被抑制的细胞因子的产生水平。这是首次有报告显示口服AHCC可增强免疫系统功能,从而增加宿主对衣原体生殖器感染的抵抗力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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