Analysis of novel prognostic markers of oral squamous cell carcinoma at the transcriptome-wide level

IF 1 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES
Li Kunshan, Liu Yuanhang, Sun Jieming, Qiu Junping, Wang Wenjing, Qiu Yongle
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引用次数: 0

Abstract

Patients with oral squamous cell carcinoma (OSCC) have a poor prognosis. The molecular mechanism of OSCC progression remains unclear. The aim of this study was to explore specific prognostic biomarkers of OSCC at the transcriptome-wide level. A total of 158 differentially expressed long non-coding RNAs (lncRNAs), including 85 cis-acting and 10 trans-acting differentially expressed lncRNAs, were screened in OSCC tissues compared with adjacent normal tissues. Both cisand trans-target genes of differentially expressed lncRNAs were enriched in pathways associated with cancer progression. Additionally, 25 differentially expressed mRNAs were screened. Overlapping analysis of differential protein-coding genes with the cisand trans-target genes of differentially expressed lncRNAs indicated that KIF19 was the only trans-acting gene of differentially expressed lncRNA ‘ENST00000626052’ (named ENSTa). ENSTa was down-regulated in OSCC tissues and cells, and its low expression corresponded to a poor prognosis in OSCC. KIF19 was highly expressed in OSCC tissues and cells. Patients with high expression of KIF19 had a poor prognosis. Cellular experiments showed that KIF19 expression and cell proliferation were inhibited in cells overexpressing ENSTa. However, KIF19 overexpression counteracted the effect of ENSTa overexpression on cell proliferation. In conclusion, ENSTa regulated the trans-acting KIF19 gene in the inhibition of the progression of OSCC, thus they could be suggested as novel biomarkers for OSCC.
在转录组水平上分析口腔鳞状细胞癌新的预后标志物
口腔鳞状细胞癌(OSCC)患者预后较差。OSCC进展的分子机制尚不清楚。本研究的目的是在转录组水平上探索OSCC的特异性预后生物标志物。与邻近正常组织相比,在OSCC组织中共筛选出158个差异表达的长链非编码rna (lncRNAs),其中85个为顺式作用,10个为反式作用差异表达lncRNAs。差异表达lncrna的顺式和反式靶基因在与癌症进展相关的途径中都富集。此外,还筛选了25个差异表达mrna。差异蛋白编码基因与差异表达lncRNA的顺式和反式靶基因的重叠分析表明,KIF19是差异表达lncRNA ' ENST00000626052 '(命名为ENSTa)唯一的反式基因。ENSTa在OSCC组织和细胞中表达下调,其低表达与OSCC预后不良相关。KIF19在OSCC组织和细胞中高表达。KIF19高表达患者预后较差。细胞实验表明,过表达ENSTa的细胞可抑制KIF19的表达和细胞增殖。然而,KIF19过表达抵消了ENSTa过表达对细胞增殖的影响。综上所述,ENSTa调节反式作用的KIF19基因抑制OSCC的进展,因此可以建议它们作为OSCC的新型生物标志物。
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来源期刊
Scienceasia
Scienceasia MULTIDISCIPLINARY SCIENCES-
CiteScore
1.70
自引率
33.30%
发文量
102
审稿时长
1 months
期刊介绍: ScienceAsia is a multidisciplinary journal publishing papers of high quality bimonthly, in printed and electronic versions, by the Science Society of Thailand under Royal Patronage and the National Research Council of Thailand. The journal publishes original research papers that provide novel findings and important contribution to broad area in science and mathematics. Areas covered include Biological Sciences and Biotechnology, Chemistry and Material Sciences, Environmental and Applied Sciences, and Mathematics and Physical Sciences. Manuscripts may report scientifically useful data, observations or model predictions, and/or provide a new scientific concept or a new explanation of published results. Submissions of materials of current scientific interest are highly welcome, provided that there is sufficient scientific merit. The journal will not accept manuscripts which have been published or are being considered for publication elsewhere, nor should manuscripts being considered by ScienceAsia be submitted to other journals. Submitted manuscripts must conform to the guidelines given in the Instructions for Authors
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