The RP-HPLC method for analysis of usnic acid as potential marker of herbal drugs-based formulations containing Usnea barbata

IF 1 4区化学 Q4 CHEMISTRY, MULTIDISCIPLINARY

Journal of The Serbian Chemical Society Pub Date : 2022-01-01 DOI:10.2298/jsc201216045t

V. Tadić, A. Zugic, S. Djordjevic, I. Zizović, I. Homšek, D. Mišić, I. Nešić

引用次数: 3

Abstract

The aim of this study was to develop and validate sensitive RP-HPLC method for determination of usnic acid, as potential marker substance of the herbal product for oromucosal use being the complex mixture of plant extracts and an essential oil, including the extract of Usnea barbata. Analysis of usnic acid in the tested formulation was performed through an extraction with methanol, prior to injection on the HPLC column (Zorbax Eclipse XDB-C18 600 Bar (4.6?100 mm, 1.8 ?m)). Employed gradient procedure of the solvents (phosphoric acid (pH 2.5) and acetonitrile), at flow rate of 0.1 mL/min allowed efficient and reproducible separation of usnic acid from other compounds present in the investigated complex mixture. The established suitability, linearity, precision, accuracy and selectivity/specificity of this assay implied its applicability as a reliable examination in the stability study of the investigated multi-ingredient herbal preparation.

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采用反相高效液相色谱法分析山茱萸中药制剂中山茱萸酸的潜在标记物

本研究的目的是建立并验证一种灵敏的反相高效液相色谱法(RP-HPLC)，用于测定山茱萸酸的含量。山茱萸酸是一种由植物提取物和精油组成的复杂混合物，其中包括山茱萸提取物。在高效液相色谱柱(Zorbax Eclipse XDB-C18 600 Bar (4.6?100毫米，1.8 ?m))。采用溶剂(磷酸(pH 2.5)和乙腈)的梯度法，在0.1 mL/min的流速下，可以从所研究的复杂混合物中有效地分离出usic酸和其他化合物。该方法的适宜性、线性度、精密度、准确度和选择性/特异性表明其可作为一种可靠的检测方法用于所研究的多成分中药制剂的稳定性研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of The Serbian Chemical Society 化学-化学综合

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of the Serbian Chemical Society -JSCS (formerly Glasnik Hemijskog društva Beograd) publishes articles original papers that have not been published previously, from the fields of fundamental and applied chemistry: Theoretical Chemistry, Organic Chemistry, Biochemistry and Biotechnology, Food Chemistry, Technology and Engineering, Inorganic Chemistry, Polymers, Analytical Chemistry, Physical Chemistry, Spectroscopy, Electrochemistry, Thermodynamics, Chemical Engineering, Textile Engineering, Materials, Ceramics, Metallurgy, Geochemistry, Environmental Chemistry, History of and Education in Chemistry.