HPLC-NNE 13 CNMR coupling fingerprint analysis technology and its application in a study of Syringa pubescens Turcz and its activity against hepatic fibrosis

Abstract

This study describes the active ingredients of Syringa pubescens Turcz which has been identified as being able to protect against hepatic fibrosis. Here we report the characteris - tics of high performance liquid chromatography and non-nuclear overhauser effect carbon-13 nuclear magnetic resonance (HPLC-NNE 13 CNMR) technology developed for coupling finger - print analysis. The major contribution of this new method is the development of an efficient technology and a useful tool for analysis of a traditional Chinese herbal medicine using chro- matography and spectral coupling fingerprint technology. Isolation of secoiridoid glycosides and investigation of their structure-activity relationship showed that these derivatives are the active ingredients of Syringa pubescens Turcz, and account for the activity of this plant against hepatic fibrosis. The active compounds were identified as oleuropein, 10-hydroxyoleuropein, oleoside-11-methylester, (8Z)-ligstroside, and echinacoside by HPLC-NNE 13 CNMR coupling fingerprint analysis. A concentration-response relationship was also demonstrated for the HPLC- NNE 13 CNMR coupling fingerprint method.