Genetic characterization and virus isolation of porcine reproductive and respiratory syndrome virus

IF 0.4 4区农林科学 Q4 VETERINARY SCIENCES

Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice Pub Date : 2023-01-01 DOI:10.21521/mw.6789

Yaping Chen, Zhengliang Liu, Dongyu Liu

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引用次数: 0

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) is an acute, febrile, highly contagious infectious disease characterized by reproductive dysfunction and respiratory failure. Its high morbidity and mortality have caused significant economic losses to the swine industry. With the continuous development of PRRSV, its genetic variation is also increasing, which causes great difficulties for the clinical prevention and control of infections casued by the virus. Monitoring the genetic variation of the virus and vaccine immunity at any time is the current research focus. In this study, patients suspected of having PRRSV were sampled, and the virus was successfully isolated. The genomic variation characteristics of the new isolate of HLJ03 were determined, especially the molecular characteristics of GP5 and NSP2, and the main focus was placed on the analysis of genetic variation. The results provide important information for further investigation of the molecular prevalence of porcine PRRSV and create a basis for the prevention and control of porcine PRRSV infections.

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猪繁殖与呼吸综合征病毒的遗传特征及病毒分离

猪繁殖与呼吸综合征病毒(PRRSV)是一种以生殖功能障碍和呼吸衰竭为特征的急性、发热、高度传染性传染病。它的高发病率和死亡率给养猪业造成了重大的经济损失。随着PRRSV的不断发展，其遗传变异也在不断增加，这给临床预防和控制由该病毒引起的感染带来了很大的困难。随时监测病毒的遗传变异和疫苗免疫是目前的研究重点。本研究对疑似感染PRRSV的患者取样，成功分离出病毒。确定了新分离株HLJ03的基因组变异特征，特别是GP5和NSP2的分子特征，重点进行了遗传变异分析。该结果为进一步研究猪PRRSV的分子流行病学提供了重要信息，为预防和控制猪PRRSV感染奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice VETERINARY SCIENCES-

CiteScore

0.80

自引率

0.00%

发文量

审稿时长

4-8 weeks

期刊介绍： "Medycyna Weterynaryjna" publishes various types of articles which are grouped in the following editorial categories: reviews, original studies, scientific and professional problems, the history of veterinary medicine, posthumous memoirs, as well as chronicles that briefly relate scientific advances and developments in the veterinary profession and medicine. The most important are the first two categories, which are published with short summaries in English. Moreover, from 2001 the editors of "Medycyna Weterynaryjna", bearing in mind market demands, has also started publishing entire works in English. Since 2008 the periodical has appeared in an electronic version. The following are available in this version: summaries of studies published from 1999 to 2005, full versions of all the studies published in the years 2006-2011 (in pdf files), and full versions of the English studies published in the current year (pdf). Only summaries of the remaining studies from the current year are available. In accordance with the principles accepted by the editors, the full versions of these texts will not be made available until next year. All articles are evaluated twice by leading Polish scientists and professionals before they are considered for publication. For years now "Medycyna Weterynaryjna" has maintained a high standard thanks to this system. The review articles are actually succinct monographs dealing with specific scientific and professional problems that are based on the most recent findings. Original works have a particular value, since they present research carried out in Polish and international scientific centers.