Geographic Differences in Mitochondrial DNA (mtDNA) Distribution Among United States (US) Domestic Dog Populations

The open forensic science journal Pub Date : 2012-01-01 DOI:10.2174/1874402801003010022

Briana B. Smalling, J. Satkoski, Bradley K. Tom, W. Szeto, Bethany Joy-Alise Erickson, T. Spear, D. G. Smith, B. Budowle, K. Webb, M. Allard, S. Kanthaswamy

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引用次数: 7

Abstract

No geographic differences in mitochondrial DNA (mtDNA) distribution among United States (US) domestic dog populations have been detected to date. To test the hypothesis that regional differences exist, a 608 bp sequence of the canid mtDNA hypervariable region 1 (HV1) from 220 mixed breed animals from the Western, Northeastern, Midwestern, and Southern US were combined with 429 published mixed and pure breed dog HV1 sequences to form a substantial geographically representative dataset. With an increased sample size of regionally representative sequences, geographic substructure among regional populations was shown to be statistically significant using the modified Fisher's exact test and pairwise Fst. The results of the AMOVA showed that 91% of the variation is present within the regional dog populations. Based on these analyses, the significance of regional canine HV1 haplotype distributions and frequencies demonstrate further the value of regional and mixed breed canine mtDNA in forensic investigations in the US.

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美国(US)家狗种群线粒体DNA (mtDNA)分布的地理差异

迄今为止，在美国(US)家犬种群中未发现线粒体DNA (mtDNA)分布的地理差异。为了验证区域差异存在的假设，我们将来自美国西部、东北部、中西部和南部220只混合品种动物的犬科动物mtDNA高可变区1 (HV1)的608 bp序列与429个已发表的混合和纯种犬HV1序列相结合，形成了一个具有地理代表性的数据集。随着区域代表性序列样本量的增加，利用改进的Fisher精确检验和两两Fst检验表明，区域种群之间的地理子结构具有统计学显著性。AMOVA的结果显示，91%的变异存在于区域犬群中。基于这些分析，犬HV1单倍型区域分布和频率的重要性进一步证明了犬mtDNA在美国法医调查中的价值。

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The open forensic science journal

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