Genetic Diversity in Dengue Mosquito, Aedes aegypti (Diptera: Culicidae) from Amazon Region: Comparative Analysis with Isozymes and RAPD Loci

J. M. Santos, E. Fraga, J. D. F. Maia, W. Tadei
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引用次数: 14

Abstract

Four populations of Aedes aegypti from Manaus were studied, using allozymes and RAPD loci, to determine intra- and interpopulation genetic variability and differentiation and to compare genetic structure parameters assessed with both markers. Five RAPD primers produced 52 polymorphic fragments, whereas only seven of 18 isozyme loci were polymorphic. The population from Praca 14 was the most polymorphic (P= 94.23% and P= 55.6%); while those from Coroado (P= 82.69% and P= 44.40%) and from Cidade Nova (P= 84.61% and P= 44.40%) were the least polymorphic, for both RAPD and isozymes respectively. The observed heterozygosity was higher between populations (Ho= 0.33 - 0.38) as assessed by RAPD. Wright's F statistics showed an F is value higher than F st (F is = 0.164 > F st = 0.048). AMOVA indicated that 95.12% of the genetic variability is intrapopulational. Even so, both of the genetic markers evaluated showed a relatively high gene flow ((N m = 15.15), and possibly are still random couplings, although the F is value was not low. The genetic distance between populations was similarly low for both markers: RAPD (0.012 - 0.016) and Isozymes (0.003 - 0.016). These results show that as assessed by both markers, the populations are genetically similar, and that isozymes (codominant) are the most efficient to detect the population genetic structure. Although isozymes revealed less genetic diversity than RAPDs, the estimated levels of genetic distance were identical.
亚马逊地区埃及伊蚊(双翅目:库蚊科)的遗传多样性:同工酶和RAPD位点的比较分析
利用同工酶和RAPD基因座对来自马瑙斯的4个埃及伊蚊种群进行研究,分析种群内和种群间的遗传变异和分化,并比较两种标记评估的遗传结构参数。5条RAPD引物产生52个多态性片段,而18个同工酶位点中只有7个是多态性的。prac14居群多态性最高(P= 94.23%和P= 55.6%);而来自Coroado (P= 82.69%和P= 44.40%)和Cidade Nova (P= 84.61%和P= 44.40%)的RAPD和同工酶多态性最低。RAPD结果表明,群体间杂合度较高(Ho= 0.33 ~ 0.38)。Wright的F统计量显示,F is值高于F st (F is = 0.164 > F st = 0.048)。AMOVA分析表明,95.12%的遗传变异发生在种群内。尽管如此,所评估的两个遗传标记均表现出较高的基因流((N m = 15.15),尽管F is值并不低,但仍可能是随机偶联。RAPD(0.012 ~ 0.016)和同工酶(0.003 ~ 0.016)两种标记的群体间遗传距离均较低。结果表明,两种标记均显示群体遗传相似,同工酶(共显性)是检测群体遗传结构最有效的方法。虽然同工酶的遗传多样性低于rapd,但估计的遗传距离水平是相同的。
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