Fluorogenic Reactions in Chemical Biology: Seeing Chemistry in Cells

Chemical & Biomedical Imaging Pub Date : 2023-05-30 DOI:10.1021/cbmi.3c00029

Yanyan Chen, Hao Jiang, Tingting Hao, Nan Zhang, Mingyu Li, Xingyun Wang, Xiuxiu Wang*, Wei Wei* and Jing Zhao*,

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Abstract

Among the recent development of click chemistry and bioorthogonal chemistry, fluorogenic reactions occupy a unique place in that fluorescence is generated from nonfluorescent reactants, thereby rendering them highly useful and convenient in no-wash live-cell imaging. This topic was extensively reviewed in 2010 by Wang et al. ( Chem. Soc. Rev. 2010, 39, 1233−1239) and in 2014 by Lin et al. ( Curr. Opin. Chem. Biol. 2014, 21, 89−95). This review presents a comprehensive and up-to-date overview on the fluorogenic reactions in the past decade. The reactions are classified into four major categories on the basis of the mechanisms of fluorescence generation. Representative examples of each type are discussed briefly in terms of structure, mechanism, and advantages. We describe the latest applications of fluorogenic reactions in chemical biology. In the end, future opportunities and challenges in this field are tentatively proposed.

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化学生物学中的荧光反应：看细胞中的化学

在点击化学和生物正交化学的最新发展中，荧光反应占据了独特的位置，因为荧光是由非荧光反应物产生的，从而使它们在无冲洗活细胞成像中非常有用和方便。2010年，王等人（Chem.Soc.Rev.2010，391233-1239）和2014年，林等人（Curr.Opin.Chem.Biol.2014，21189-95）对该主题进行了广泛审查。这篇综述对过去十年中的荧光反应进行了全面和最新的综述。根据荧光产生的机制，将反应分为四大类。从结构、机理和优点等方面简要讨论了每种类型的代表性实例。我们介绍了荧光反应在化学生物学中的最新应用。最后，初步提出了该领域未来的机遇和挑战。

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来源期刊

Chemical & Biomedical Imaging 化学与生物成像-

CiteScore

1.00

自引率

0.00%

发文量

期刊介绍： Chemical & Biomedical Imaging is a peer-reviewed open access journal devoted to the publication of cutting-edge research papers on all aspects of chemical and biomedical imaging. This interdisciplinary field sits at the intersection of chemistry physics biology materials engineering and medicine. The journal aims to bring together researchers from across these disciplines to address cutting-edge challenges of fundamental research and applications.Topics of particular interest include but are not limited to:Imaging of processes and reactionsImaging of nanoscale microscale and mesoscale materialsImaging of biological interactions and interfacesSingle-molecule and cellular imagingWhole-organ and whole-body imagingMolecular imaging probes and contrast agentsBioluminescence chemiluminescence and electrochemiluminescence imagingNanophotonics and imagingChemical tools for new imaging modalitiesChemical and imaging techniques in diagnosis and therapyImaging-guided drug deliveryAI and machine learning assisted imaging