{"title":"Structural Analysis and Phylogenetic Relationships of a Teleost Fish, Pethia stoliczkana Based on the Complete Mitochondrial Genome Sequence","authors":"Z. Qiao","doi":"10.17582/journal.pjz/20230302100304","DOIUrl":null,"url":null,"abstract":"In this study, the whole mitochondrial genome sequence of Pethia stoliczkana was obtained using high-throughput sequencing technology, and its structure and characteristics were analyzed. The P. stoliczkana mitochondrial genome contained a total of 16,966 base pairs, including 13 protein-coding genes, 22 transport RNA genes, two ribosomal RNA genes, and one control region. The A+T content (59.7%) of the whole mitochondrial genome was greater than the G+C content (40.3%), indicating an obvious A+T preference. The mitochondrial genome of P. stoliczkana is similar to that of most teleost fish, and no gene rearrangements were detected. The phylogenetic relationship tree of Smiliogastrinae fish was constructed based on 13 protein-coding genes using the Bayesian inference and maximum likelihood methods. We found that P. stoliczkana was closely related to Pethia ticto and Pethia padamya . These results enrich the mitochondrial genome database of Smiliogastrinae fish and provide reference materials for systematic classification of this group of fish.","PeriodicalId":19975,"journal":{"name":"Pakistan Journal of Zoology","volume":null,"pages":null},"PeriodicalIF":0.5000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pakistan Journal of Zoology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.17582/journal.pjz/20230302100304","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ZOOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
In this study, the whole mitochondrial genome sequence of Pethia stoliczkana was obtained using high-throughput sequencing technology, and its structure and characteristics were analyzed. The P. stoliczkana mitochondrial genome contained a total of 16,966 base pairs, including 13 protein-coding genes, 22 transport RNA genes, two ribosomal RNA genes, and one control region. The A+T content (59.7%) of the whole mitochondrial genome was greater than the G+C content (40.3%), indicating an obvious A+T preference. The mitochondrial genome of P. stoliczkana is similar to that of most teleost fish, and no gene rearrangements were detected. The phylogenetic relationship tree of Smiliogastrinae fish was constructed based on 13 protein-coding genes using the Bayesian inference and maximum likelihood methods. We found that P. stoliczkana was closely related to Pethia ticto and Pethia padamya . These results enrich the mitochondrial genome database of Smiliogastrinae fish and provide reference materials for systematic classification of this group of fish.
期刊介绍:
Pakistan Journal of Zoology (Pakistan J. Zool.) publishes original articles in English on all aspects of animal life. Generally these articles will be in, or related to one of the following subject areas: Physiology, Cell Biology, Molecular Biology, Genetics, Bioinformatics, Toxicology, Forensic Science, Developmental Biology, Entomology, Parasitology, Microbiology, Biotechnology, Pathology, Palaeontology. Taxonomy, Environmental Biology, Wildlife, Fisheries, Vertebrate and Invertebrate Morphology. Additionally, the journal considers research on health and clinical studies. Short communications are regularly considered, however, uninvited review articles, first records/reports of known species, case reports/studies and survey reports are not published in Pakistan Journal of Zoology.