MICROBIAL POPULATION OF HOT SPRING WATERS IN ESKİŞEHİR/TURKEY

ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology Pub Date : 2012-03-21 DOI:10.18036/BTDC.91655

N. Y. Sarıözlü, Rasime Demirel, M. Kıvanç

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引用次数: 2

Abstract

In order to investigate and find out the bacterial community of hot spring waters in Eskisehir, Turkey, 7 hot spring water samples were collected from 7 different hot springs. All samples were inoculated using four different media (nutrient agar, water yeast extract agar, trypticase soy agar, starch casein agar). After incubation at 50 oC for 14 days, all bacterial colonies were counted and purified. Gram reaction, catalase and oxidase properties of all isolates were determined and investigated by BIOLOG, VITEK and automated ribotyping system (RiboPrinter). The resistance of these bacteria was examined against ampiciline, gentamisine, trimethoprime-sulphamethoxazole and tetracycline. As a result, heat resistant pathogenic microorganisms in addition to human normal flora were determined in hot spring waters (43-50 oC) in investigated area. Ten different species belong to 6 genera were identified as Alysiella filiformis, Bordetella bronchiseptica, B. pertussis, Molexalla caprae, M. caviae, M. cuniculi, M. phenylpyruvica, Roseomonas fauriae, Delftia acidovorans and Pseudomonas taetrolens.

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eskİŞehİr /土耳其温泉的微生物种群

为了调查和了解土耳其埃斯基谢希尔地区温泉水体的细菌群落，从7个不同的温泉中采集了7份温泉样品。所有样品分别接种4种培养基(营养琼脂、水酵母膏琼脂、胰酶大豆琼脂、淀粉酪蛋白琼脂)。在50℃孵育14天后，计数并纯化所有菌落。通过BIOLOG、VITEK和RiboPrinter自动核糖分型系统(RiboPrinter)检测和研究所有分离株的革兰氏反应、过氧化氢酶和氧化酶性质。检测了这些细菌对氨苄西林、庆大霉素、甲氧嘧啶-磺胺甲恶唑和四环素的耐药性。结果，在调查区43 ~ 50℃的温泉水中，除检出人体正常菌群外，还检出了耐热性病原微生物。鉴定出丝状Alysiella、支气管脓毒杆菌、百日咳双歧杆菌、caprae Molexalla、caviae M.、ccululi M.、苯丙酮M.、fauriae玫瑰单胞菌、Delftia acidovorans和tatrolens假单胞菌6属10种。

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ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology

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审稿时长

26 weeks