EFFECT OF EXOGENOUSLY APPLIED SPERMINE AND PUTRESCINE ON GERMINATION AND IN VITRO GROWTH OF PEPPER (CAPSICUM ANNUUM L.) SEEDS UNDER SALT STRESS

Abstract

Plant growth regulators play a significant role in germination of seed. In this study,  Effects of exogenous polyamines (spermine and putrescine) on germination of seed and seedling growth such as radicula length, hypocotile length, fresh weight, dry weight of pepper under salt stress were investigated. According to results, increased salt concentration resulted in a significant reduction in germination (P<0.05). NaCl prevented hypocotile growth and this inhibition was reversed by applying spermine. During application of 2 mM spermine + 200 mM NaCl (P<0.05),  the best germination was recorded. On the contrary, germination of seed was inhibited by single application of Put but hypocotile and radicula length of seedling were increased seriously by application of 0.01 mM putrescine + 200mM NaCl and 1mM putrescine + 50 mM NaCl (P<0.05) in pepper plant.