Impedimetric label-free detection of salivary EGFR on screen printed electrode

Abstract

In this paper we present the preliminary results on the development of an electrochemical gold integrated SPE biosensor for the detection of EGFR. The strategy followed concerned the biofunctionalization of the gold electrode through the in-situ electrochemical deposition of 4-carboxymethylanailine (CMA) then the grafting of AntiEGFR antibodoy. The biosensor developed was characterized using electrochemical impedance spectroscopy (EIS) and as a result it was found to be highly sensitive within a range of 50-120 pg/mL in phosphate buffer saline (PBS) as well as very selective toward the EGFR when compared to other interferences such as HER2 and HER3.