Antibacterial effect of an extract of the endophytic fungus Alternaria alternata and its cytotoxic activity on MCF-7 and MDA MB-231 tumour cell lines

Q3 Agricultural and Biological Sciences
U.S. EZHIL ARIVUDAINAMBI, A. Kanugula, S. Kotamraju, C. Karunakaran, A. Rajendran
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引用次数: 10

Abstract

Abstract There is a growing need for new and effective antimicrobial agents to treat life-threatening diseases. Fungal endophytes are receiving increasing attention by natural product chemists due to the diverse and structurally unprecedented compounds, which make them interesting candidates for drug discovery. The present study evaluates the antibacterial activity of ethyl acetate extract of the endophytic fungus Alternaria alternata VN3 on multi-resistant clinical strains of Staphylococcus aureus and Pseudomonas aeruginosa, as well as its cytotoxicity on MCF-7 and MDA MB-231 tumour cell lines of breast cancer. The maximum inhibition zone of 21.4±0.07 mm and 21.5±0.25 mm was observed for S. aureus strain 10 and P. aeruginosa strain 2, respectively. The ethyl acetate extract showed minimal inhibitory concentration ranging from 100 to 900 μg/ml for S. aureus and P. aeruginosa. Further, the ethyl acetate extract of A. alternata VN3 exhibited moderate anticancer activity against MCF-7 and MDA MB-231 cell lines. At 30 μg/ml the cell viability was decreased to 75.5% and 71.8% for MCF-7 and MDA MB-231 cells, respectively. These results clearly indicate that the metabolites of A. alternata VN3 are a potential source for production of new drugs.
内生真菌alternnaria alternata提取物对MCF-7和MDA MB-231肿瘤细胞株的抑菌作用及其细胞毒活性
人们越来越需要新的有效的抗菌药物来治疗危及生命的疾病。真菌内生菌因其丰富多样且结构上前所未有的化合物而受到天然产物化学家越来越多的关注,这使它们成为药物发现的有趣候选者。本研究评价了内生真菌alternnaria alternata VN3乙酸乙酯提取物对临床多耐药金黄色葡萄球菌和铜绿假单胞菌的抑菌活性,以及对乳腺癌肿瘤细胞株MCF-7和MDA MB-231的细胞毒性。金黄色葡萄球菌菌株10和铜绿假单胞菌菌株2的最大抑菌带分别为21.4±0.07 mm和21.5±0.25 mm。乙酸乙酯提取物对金黄色葡萄球菌和铜绿假单胞菌的最低抑菌浓度为100 ~ 900 μg/ml。此外,竹叶莲VN3乙酸乙酯提取物对MCF-7和MDA MB-231细胞株具有中等的抗癌活性。在30 μg/ml浓度下,MCF-7和MDA MB-231细胞存活率分别降至75.5%和71.8%。这些结果清楚地表明,交替藤VN3的代谢物是生产新药的潜在来源。
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来源期刊
Biological Letters
Biological Letters Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
自引率
0.00%
发文量
0
审稿时长
52 weeks
期刊介绍: It covers a wide spectrum of biological disciplines, including animal science and zoology, general biochemistry, botany, and ecology. We also encourage submission of manuscripts related to conservation biology and wildlife management.
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