Angiogenic Effect of Bioactive Borate Glass Microfibers and Beads in the Hairless Mouse

Q1 Materials Science

Biomedical Glasses Pub Date : 2015-12-24 DOI:10.1515/bglass-2015-0017

R. J. Watters, Roger F. R. Brown, D. Day

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引用次数: 6

Abstract

Abstract The purpose of this project was to investigate the angiogenic mechanism of bioactive borate glass for soft tissue repair in a ‘hairless’ SKH1 mouse model. Subcutaneous microvascular responses to bioactive glass microfibers (45S5, 13-93B3, and 13-93B3Cu) and bioactive glass beads (13-93, 13-93B3, and 13-93B3Cu) were assessed via: noninvasive imaging of skin microvasculature; histomorphometry of microvascular densities; and quantitative PCR measurements of mRNA expression of VEGF and FGF-2 cytokines. Live imaging via dorsal skin windows showed the formation at twoweeks of a halo-like structure infused with microvessels surrounding implanted boratebased 13-93B3 and 13-93B3Cu glass beads, a response not observed with silicate-based 13-93 glass beads. Quantitative histomorphometry of tissues implanted with plugs of 45S5, 13-93B3, and 13-93B3Cu glass microfibers revealed microvascular densities that were 1.6-, 2.3-, and 2.7-times higher, respectively, than the sham control valueswhereas 13-93, 13-93B3, and 13-93B3Cu glass beads caused the microvascular density to increase 1.3-, 1.6-, and 2.5-fold, respectively, relative to sham controls. Quantitative PCR measurements indicate a marginally significant increased expression of VEGF mRNA in tissues with 13-93B3Cu glass beads, an outcome that supported the hypothesis that copper-doped borate glass could promote VEGF expression followed by angiogenesis for enhanced wound healing.

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生物活性硼酸玻璃微纤维和微珠对无毛小鼠血管生成的影响

本项目旨在研究生物活性硼酸盐玻璃在“无毛”SKH1小鼠模型中用于软组织修复的血管生成机制。皮下微血管对生物活性玻璃微纤维(45S5、13-93B3和13-93B3Cu)和生物活性玻璃微珠(13-93、13-93B3和13-93B3Cu)的反应通过以下方式评估:皮肤微血管无创成像;微血管密度组织形态学;定量PCR检测VEGF和FGF-2细胞因子mRNA表达。通过背部皮肤窗口的实时成像显示，两周后，植入的硼酸盐基13-93B3和13-93B3Cu玻璃珠周围形成了一个充满微血管的晕状结构，而硅酸盐基13-93玻璃珠则没有观察到这种反应。对植入45S5、13-93B3和13-93B3Cu玻璃微纤维的组织进行定量组织形态学分析，结果显示，微血管密度分别比假手术对照组高1.6倍、2.3倍和2.7倍，而13-93、13-93B3和13-93B3Cu玻璃微珠的组织微血管密度分别比假手术对照组高1.3倍、1.6倍和2.5倍。定量PCR检测结果显示，在13-93B3Cu玻璃微珠的组织中，VEGF mRNA的表达略有显著增加，这一结果支持了铜掺杂硼酸盐玻璃可以促进VEGF表达和血管生成，从而促进伤口愈合的假设。

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来源期刊

Biomedical Glasses Materials Science-Surfaces, Coatings and Films

自引率

0.00%

发文量

审稿时长

17 weeks

期刊介绍： Biomedical Glasses is an international Open Access-only journal covering the field of glasses for biomedical applications. The scope of the journal covers the science and technology of glasses and glass-based materials intended for applications in medicine and dentistry. It includes: Chemistry, physics, structure, design and characterization of biomedical glasses Surface science and interactions of biomedical glasses with aqueous and biological media Modeling structure and reactivity of biomedical glasses and their interfaces Biocompatibility of biomedical glasses Processing of biomedical glasses to achieve specific forms and functionality Biomedical glass coatings and composites In vitro and in vivo evaluation of biomedical glasses Glasses and glass-ceramics in engineered regeneration of tissues and organs Glass-based devices for medical and dental applications Application of glasses and glass-ceramics in healthcare.