EVALUATION OF THE LYTIC KINETICS OF A COCKTAIL OF TWO WASTEWATER SIPHOVIRIDAE BACTERIOPHAGES AGAINST UROPATHOGENIC KLEBSIELLA PNEUMONIAE

IF 0.6 4区环境科学与生态学 Q4 ECOLOGY

Applied Ecology and Environmental Research Pub Date : 2023-01-01 DOI:10.15666/aeer/2103_26832698

R. Al-Hindi, A. Bahieldin, S. Edris, I. Alotibi, S. Azhari, M. Alharbi

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Abstract

. In this study, two lytic phages were isolated which were designated as KPO1 and KPO2. These phages formed medium plaques with different size and had a head with a diameter of 54 ± 3.0 and 56 and ± 3.0 nm, respectively. They had a flexible, long noncontractile tail with a length of 85 ± 3.0 and 160 ± 3.0 nm, respectively. Both belong to order Caudovirales family Siphoviridae . They were stable at 4°C to 70°C upon thermal exposure and not viable at 80 o C and 90 o C, respectively. Concerning the pH stability, both retained a high titer (average: 8.1 log 10 PFU/mL) from pH 4 to pH 10 for 1 h. No viable virions were detected at pH 12 and pH 2. The burst size of KPO1 and KPO2 computed as 98 and 110 virions per infected cells with the corresponding latent period of 23 min and 15 min, respectively. The in vitro lytic kinetics of the cocktail of KPO1 and KPO2 was evaluated against Klebsiella pneumoniae strain INF079. The highest reduction of K. pneumoniae were observed at 0.1MOI for both phages. According to the findings, the prepared phage cocktail significantly reduced the bacterial concentration from 0.4 to 0.12 at 38 h of post infection ( p < 0.05). Hence, phages KPO1 and KPO2 could be a promising therapeutic agent for the urinary tract infections caused by K. pneumoniae .

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两种污水中虹膜病毒科噬菌体混合物对尿路致病性肺炎克雷伯菌的溶解动力学评价

。本研究分离到两个裂解噬菌体，分别命名为KPO1和KPO2。这些噬菌体形成不同大小的中等斑块，头直径分别为54±3.0 nm和56±3.0 nm。尾巴长而柔软，不收缩，长度分别为85±3.0 nm和160±3.0 nm。两者都属于尾状病毒目蚊科。它们在4 ~ 70℃热暴露时稳定，在80℃和90℃热暴露时不活。pH稳定性方面，从pH 4到pH 10，均保持高滴度(平均:8.1 log 10 PFU/mL)，在pH 12和pH 2下均未检测到活病毒体。KPO1和KPO2的爆发大小分别为每个感染细胞98和110个病毒粒子，潜伏期分别为23 min和15 min。研究了KPO1和KPO2混合物对肺炎克雷伯菌INF079的体外裂解动力学。两种噬菌体在0.1MOI时均观察到肺炎克雷伯菌的最大减少。结果表明，在感染后38 h，制备的噬菌体鸡尾酒显著降低了细菌浓度，从0.4降至0.12 (p < 0.05)。因此，噬菌体KPO1和KPO2可能是治疗肺炎克雷伯菌引起的尿路感染的一种有前景的药物。

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来源期刊

Applied Ecology and Environmental Research ECOLOGY-ENVIRONMENTAL SCIENCES

CiteScore

1.40

自引率

14.30%

发文量

104

审稿时长

14 months

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