DIFFERENCES OF COPEPODA COMPOSITION ANALYSIS IN MARICULTURE PONDS THROUGH MORPHOLOGICAL TAXONOMY AND DIFFERENT METAGENETIC APPROACHES

IF 0.6 4区环境科学与生态学 Q4 ECOLOGY

Applied Ecology and Environmental Research Pub Date : 2023-01-01 DOI:10.15666/aeer/2103_20912131

S. Wu, X. Zhang, L. Dai, J. Ni, Y. Wang, J.J. Li, W. Ding

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引用次数: 0

Abstract

. Copepods play a fundamental role in aquatic ecosystems and are an important food source for fish at an early stage. However, little attention has been given to changes in copepod communities in aquaculture water. This is mainly limited by the copepod analysis method. In this study, we designed a pair of copepod primers, Cop18SF/Cop18SR, for the analysis of copepod communities to promote the investigation of copepods in aquaculture water. Our results showed that the common-used primer pair Uni18S/Uni18SR non-specifically amplified many DNA sequences from bacteria, whereas the primer pair LSUCop-D2F/LSUCop-D2R abnormally enriched OTU denovo438, which was an unidentified Eukaryota zooplankton. Although Eurytemora pacifica was classified into 26 OTUs with 97% sequence identity using our designed primer pair Cop18SF/Cop18SR, other results indicated that the primer pair Cop18SF/Cop18SR was superior to the other two primer pairs. Moreover, different primer pairs yielded different plankton community structures, which caused their correlations with environmental factors to be obviously different. These results imply that the primer pair Cop18SF/Cop18SR can be used to analyze the dynamic changes in the copepod community structure and the relationship between the community structure and abiotic and biological factors in aquaculture water.

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形态分类学及不同元生方法分析海水养殖池塘桡足类组成差异

。桡足类动物在水生生态系统中起着基础性的作用，是鱼类早期的重要食物来源。然而，对养殖水体中桡足动物群落的变化关注甚少。这主要受到桡足类分析方法的限制。本研究设计了Cop18SF/Cop18SR对桡足动物群落分析引物，以促进对养殖水体桡足动物的调查。结果表明，常用的引物Uni18S/Uni18SR非特异性扩增了许多细菌DNA序列，而引物LSUCop-D2F/LSUCop-D2R异常富集了未知真核浮游动物OTU denovo438。虽然我们设计的引物对Cop18SF/Cop18SR将太平洋Eurytemora pacifica分类为26个otu，序列同源性为97%，但其他结果表明，引物对Cop18SF/Cop18SR优于其他两个引物对。此外，不同引物对产生的浮游生物群落结构也不同，导致它们与环境因子的相关性存在明显差异。这些结果表明，Cop18SF/Cop18SR引物对可用于分析养殖水体桡足类群落结构的动态变化以及群落结构与非生物和生物因子的关系。

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来源期刊

Applied Ecology and Environmental Research ECOLOGY-ENVIRONMENTAL SCIENCES

CiteScore

1.40

自引率

14.30%

发文量

104

审稿时长

14 months

期刊介绍： The Journal publishes original research papers and review articles. Researchers from all countries are invited to publish pure or applied ecological, environmental, biogeographical, zoological, botanical, paleontological, biometrical-biomathematical and quantitative ecological or multidisciplinary agricultural research of international interest on its pages. The focus is on topics such as: -Community, ecosystem and global ecology- Biometrics, theoretical- and quantitative ecology- Multidisciplinary agricultural and environmental research- Sustainable and organic agriculture, natural resource management- Ecological methodology, monitoring and modeling- Biodiversity and ecosystem research, microbiology, botany and zoology- Biostatistics and modeling in epidemiology, public health and veterinary- Earth history, paleontology, extinctions, biogeography, biogeochemistry- Conservation biology, environmental protection- Ecological economics, natural capital and ecosystem services- Climatology, meteorology, climate change, climate-ecology. The Journal publishes theoretical papers as well as application-oriented contributions and practical case studies. There is no bias with regard to taxon or geographical area. Purely descriptive papers (like only taxonomic lists) will not be accepted for publication.