IDENTIFICATION OF GINSENG (PANAX GINSENG C. A. MAYER) CONTINUOUS CROPPING OBSTACLE RESPONSIVE MIRNAS AND THEIR TARGET GENES

IF 0.6 4区环境科学与生态学 Q4 ECOLOGY

Applied Ecology and Environmental Research Pub Date : 2023-01-01 DOI:10.15666/aeer/2102_10251041

Y. Shen, L. Cheng, T. Zhang, Q. Li, Y. Zhan, N. Yan, E. Wang, C. Chen

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引用次数: 2

Abstract

. The problem of continuous cropping obstacle has seriously restricted the development of the ginseng industry. In view of the little research on the mechanism of ginseng crop disorder, this study was conducted to investigate the ginseng crop disorder-related miRNAs and their target genes for the sustainability of the ginseng industry. A total of 376 miRNAs were obtained from all the tissue samples of the Continuous Cropping obstacles (CCO) and Control Check (CK). 121 miRNAs were found to be differentially expressed in the ginseng. Then, the target genes of differentially expressed miRNAs were enriched and found to be involved in phytohormone-related regulation, MAPK signaling pathway - plant, amino and nucleotide sugar metabolism, and monoterpene biosynthesis, suggesting that the alteration of these related pathways may play a key role in causing ginseng crop disorder. Finally, the correlations between miRNAs and mRNAs were integrated to construct the corresponding reciprocal networks and discover the miRNAs that might play a key role in causing ginseng crop disorder. osa-MIR818d-p3_1ss1TA and pgi-MIR6135i-p3_1ss11TG and pgi-MIR6140c-p5_1ss12CT were found to be at the core of the network, and it was hypothesized that these miRNAs play an important role in the formation of ginseng continuous cropping obstacle.

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人参(panax ginseng c. a. mayer)连作障碍应答mirna及其靶基因的鉴定

．连作障碍问题严重制约了人参产业的发展。鉴于目前对人参作物病害发生机制的研究较少，本研究旨在研究人参作物病害相关mirna及其靶基因，以促进人参产业的可持续发展。从连作障碍(CCO)和对照检查(CK)的所有组织样本中共获得376个mirna。在人参中发现了121个mirna的差异表达。随后，我们富集了差异表达mirna的靶基因，发现它们参与了植物激素相关调控、MAPK信号通路-植物、氨基和核苷酸糖代谢以及单萜类生物合成，提示这些相关通路的改变可能是导致人参作物失调的关键因素。最后，整合mirna和mrna之间的相关性，构建相应的互惠网络，发现可能在人参作物病害中起关键作用的mirna。发现osa-MIR818d-p3_1ss1TA和pgi-MIR6135i-p3_1ss11TG和pgi-MIR6140c-p5_1ss12CT位于该网络的核心，推测这些mirna在人参连作障碍的形成过程中发挥重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Applied Ecology and Environmental Research ECOLOGY-ENVIRONMENTAL SCIENCES

CiteScore

1.40

自引率

14.30%

发文量

104

审稿时长

14 months

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