Biodistribution of Intravenously Transplanted Mitochondria Conjugated with Graphene Quantum Dots in Diabetic Rats.

IF 2.6 4区化学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Fluorescence Pub Date : 2024-11-01 Epub Date: 2023-10-28 DOI:10.1007/s10895-023-03480-0

Pallavi Mudgal, Jyotsna Pareek, Swati Paliwal

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引用次数: 0

Abstract

Mitochondria transplantation has emerged as a successful therapeutic modality to treat several degenerative diseases. However, the biodistribution of transplanted mitochondria has not been well studied. We investigated the ex-vivo systemic biodistribution and therapeutic efficacy of intravenously transplanted graphene quantum dots (GQDs) conjugated to isolated mitochondria (Mt-GQDs) in diabetic rat tissues. The results revealed that Mt-GQDs facilitate the tracking of transplanted mitochondria without affecting their therapeutic efficacy. It is compelling to note that Mt-GQDs and isolated mitochondria show comparable therapeutic efficacies in decreasing blood glucose levels, oxidative stress, inflammatory gene expressions, and restoration of different mitochondrial functions in pancreatic tissues of diabetic rats. In addition, histological section examination under a fluorescence microscope demonstrated the localization of Mt-GQDs in multiple tissues of diabetic rats. In conclusion, this study indicates that Mt-GQDs provide an effective mitochondrial transplantation tracking modality.

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静脉移植的石墨烯量子点结合线粒体在糖尿病大鼠中的生物分布。

线粒体移植已成为治疗几种退行性疾病的一种成功的治疗方式。然而，移植线粒体的生物分布尚未得到很好的研究。我们研究了静脉移植的与分离的线粒体结合的石墨烯量子点（GQDs）在糖尿病大鼠组织中的离体系统生物分布和治疗效果。结果表明，Mt-GQDs有助于追踪移植的线粒体，而不会影响其治疗效果。值得注意的是，Mt-GQDs和分离的线粒体在降低糖尿病大鼠血糖水平、氧化应激、炎症基因表达和恢复胰腺组织中不同线粒体功能方面表现出相当的治疗效果。此外，荧光显微镜下的组织学切片检查显示Mt-GQDs在糖尿病大鼠的多个组织中定位。总之，本研究表明Mt-GQDs提供了一种有效的线粒体移植跟踪模式。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Fluorescence 化学-分析化学

CiteScore

4.60

自引率

7.40%

发文量

203

审稿时长

5.4 months

期刊介绍： Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.