Hydrogen production by unicellular green alga chlorella sp. LSD-W2 isolated from seawater in Thailand

Q3 Agricultural and Biological Sciences

Asia-Pacific Journal of Science and Technology Pub Date : 2016-07-15 DOI:10.14456/KKURJ.2016.32

N. Tinpranee, A. Incharoensakdi, S. Phunpruch

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引用次数: 3

Abstract

Green algae are able to convert the unlimited sunlight energy to produce hydrogen via photosynthesis. In seawater, several kinds of m arine microalgae are widespread and abundant and have been shown to tolerate and survive under the extreme salt concentrations . This work aimed to study the screening of high H 2 producing marine green algal strains isolated from the Gulf of Thailand and the Andaman Sea, and the selection of the highest H 2 producing strain. Its H 2 production was investigated under photoheterotrophic cultivation. The result revealed that among 20 marine green algal strains, the green alga Chlorella sp. LSD-W2 gave the highest H 2 production rate in both light and dark anaerobic conditions. During photoheterotrophic cultivation Chlorella sp. LSD-W2 was rapidly grown in TAP (Tris-Acetate-Phosphate) medium and reached the stationary growth phase after 36 h of cultivation. The highest photohydrogen production rate was found in cells incubated in NH 4 Cl-deprived TAP medium. It was approximately 20-fold higher than H 2 production rate of cells in a normal TAP medium.

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从泰国海水中分离的单细胞绿藻小球藻sp. LSD-W2产氢

绿藻能够通过光合作用将无限的阳光能量转化为氢气。在海水中，几种海洋微藻分布广泛、数量丰富，并能在极端盐浓度下耐受和生存。本研究旨在对泰国湾和安达曼海产氢量高的海洋绿藻菌株进行筛选，并筛选出产氢量最高的菌株。对其在光异养培养下的h2产量进行了研究。结果表明，在20株海洋绿藻中，绿藻小球藻sp. LSD-W2在光照和黑暗厌氧条件下的h2产率最高。在光异养培养过程中，小球藻LSD-W2在TAP (Tris-Acetate-Phosphate)培养基中快速生长，培养36 h后达到稳生期。在缺氧的TAP培养基中培养的细胞产氢率最高。它比在正常TAP培养基中的细胞产生h2的速率高约20倍。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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