Determination of Bilirubin Using near Infrared Diffuse Reflectance Spectroscopy with Selective Concentration on β-Cyclodextrin

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2016-06-24 DOI:10.1255/jnirs.1224

Cuicui Wang, W. Cai, X. Shao

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引用次数: 8

Abstract

Near infrared diffuse reflectance spectroscopy (NIR-DRS) is a convenient and fast quantitative method for direct analysis of complex samples. The low sensitivity of NIR-DRS, however, is a problem for micro-analysis. A method for improving the sensitivity of NIR-DRS is proposed using selective adsorption. The method makes use of an adsorbent containing β-cyclodextrin to achieve the specific adsorption of bilirubin (BR). Preconcentration of BR from an aqueous solution with complex matrix was achieved through hydrophobic interaction and hydrogen bonding. Then NIR-DRS of the adsorbate was conducted and a partial least squares model was built for quantitative prediction. Both the preconcentration and the specific adsorption improve the analysis. The former enhances the spectral response by enrichment, and the latter makes the NIR response less affected by interferences such as metal ions, glucose etc. The results for the validation samples were a coefficient of determination (r2) between the predicted and reference concentration of 0.98 with recoveries in the range 94.5′-107.5% in a concentration range of 3.30′-8.60 μg mL−1. The concentration that can be quantitatively detected was as low as 2.60 μg mL−1. Considering that the normal concentration range of BR in serum is 3.5′-10.0 μg mL−1, the method may provide a feasible tool for determination of BR in serum or other solution samples.

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选择性浓度β-环糊精近红外漫反射光谱法测定胆红素

近红外漫反射光谱法(NIR-DRS)是一种方便、快速的直接分析复杂样品的定量方法。然而，NIR-DRS的低灵敏度是微观分析的一个问题。提出了一种利用选择性吸附提高NIR-DRS灵敏度的方法。该方法利用含有β-环糊精的吸附剂实现对胆红素(BR)的特异性吸附。通过疏水相互作用和氢键作用，实现了复合基质水溶液中BR的预富集。然后对吸附质进行NIR-DRS，建立偏最小二乘模型进行定量预测。预富集和特异吸附都提高了分析的准确性。前者通过富集提高了光谱响应，后者使近红外响应受金属离子、葡萄糖等干扰的影响较小。验证样品的预测浓度与标准浓度之间的决定系数(r2)为0.98，在3.30′~ 8.60 μ mL−1范围内加样回收率为94.5′~ 107.5%。可定量检测的浓度低至2.60 μg mL−1。考虑到血清中BR的正常浓度范围为3.5 μg -10.0 μg mL−1，该方法可为血清或其他溶液样品中BR的测定提供一种可行的工具。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.