Mucus secretion and collagen fibres integrity are compromised in aspirin induced gastric lesion; protective role of Musa paradisiaca

Q4 Medicine

Italian journal of anatomy and embryology Pub Date : 2018-01-01 DOI:10.13128/IJAE-24163

Alese Margaret Olutayo, O. S. Adewole, O. Ijomone, D. Ofusori, O. Alese

{"title":"Mucus secretion and collagen fibres integrity are compromised in aspirin induced gastric lesion; protective role of Musa paradisiaca","authors":"Alese Margaret Olutayo, O. S. Adewole, O. Ijomone, D. Ofusori, O. Alese","doi":"10.13128/IJAE-24163","DOIUrl":null,"url":null,"abstract":"The study investigated the healing effects of flavonoid-rich fraction of Musa paradisiaca fruit on the gastric corpus of Wistar rats following aspirin induced-gastric lesion. Ninety adult male Wistar rats were assigned to 6 groups. Gastric lesion was induced in groups B, C, D, E and F rats by administration of 400 mg/kg aspirin. After 24 hours, the extract of M. paradisiaca was administered to groups C, D and E at graded doses for 21 days. Group F rats received omeprazole at 1.8 mg/kg daily for 21 days. The rats were sacrificed on days 14, 21 and 28. Gastric tissues were fixed in neutral buffered formalin and processed by paraffin wax embedding. Sections were stained with haematoxylin and eosin, Masson’s trichrome and periodic acid-Schiff’s. There was a gradual restoration of the damaged epithelia in the treatment groups. Histomorphometric studies revealed a significant increase in the total thickness of mucosal layers in the treatment groups when compared with aspirin only group. There was a dose-dependent improvement in staining for mucins in the treatment groups. Also, the treatment groups displayed a progressively positive stain for deposition of collagen. It is concluded that M. paradisiaca significantly attenuated the damaging effects of aspirin on the gastric mucosa, probably via a mechanism involving increase in mucin secretion.","PeriodicalId":14636,"journal":{"name":"Italian journal of anatomy and embryology","volume":"123 1","pages":"136-148"},"PeriodicalIF":0.0000,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.13128/IJAE-24163","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Italian journal of anatomy and embryology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.13128/IJAE-24163","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 2

Abstract

The study investigated the healing effects of flavonoid-rich fraction of Musa paradisiaca fruit on the gastric corpus of Wistar rats following aspirin induced-gastric lesion. Ninety adult male Wistar rats were assigned to 6 groups. Gastric lesion was induced in groups B, C, D, E and F rats by administration of 400 mg/kg aspirin. After 24 hours, the extract of M. paradisiaca was administered to groups C, D and E at graded doses for 21 days. Group F rats received omeprazole at 1.8 mg/kg daily for 21 days. The rats were sacrificed on days 14, 21 and 28. Gastric tissues were fixed in neutral buffered formalin and processed by paraffin wax embedding. Sections were stained with haematoxylin and eosin, Masson’s trichrome and periodic acid-Schiff’s. There was a gradual restoration of the damaged epithelia in the treatment groups. Histomorphometric studies revealed a significant increase in the total thickness of mucosal layers in the treatment groups when compared with aspirin only group. There was a dose-dependent improvement in staining for mucins in the treatment groups. Also, the treatment groups displayed a progressively positive stain for deposition of collagen. It is concluded that M. paradisiaca significantly attenuated the damaging effects of aspirin on the gastric mucosa, probably via a mechanism involving increase in mucin secretion.

查看原文本刊更多论文

阿司匹林引起的胃病变损害了粘液分泌和胶原纤维的完整性;天堂穆萨的保护作用

研究了富黄酮提取物天麻果对阿司匹林致胃损伤大鼠胃主体的修复作用。将90只成年雄性Wistar大鼠分为6组。B、C、D、E、F组大鼠给予400mg /kg阿司匹林诱导胃损伤。24 h后，C、D、E组按分级剂量给药，连续21 D。F组大鼠给予奥美拉唑1.8 mg/kg / d，连用21 d。大鼠于第14、21、28天处死。胃组织用中性缓冲福尔马林固定，石蜡包埋处理。切片用苏木精、伊红、马松三色和周期性酸-希夫染色。治疗组受损上皮逐渐恢复。组织形态学研究显示，与仅服用阿司匹林组相比，治疗组的粘膜层总厚度显著增加。治疗组黏蛋白染色有剂量依赖性改善。同时，治疗组胶原沉积呈渐进式阳性染色。由此可见，天堂支原体明显减弱阿司匹林对胃粘膜的破坏作用，其机制可能与黏液分泌增加有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Italian journal of anatomy and embryology Medicine-Embryology

CiteScore

0.20

自引率

0.00%

发文量

期刊介绍： The Italian Journal of Anatomy and Embryology, founded in 1901 by Giulio Chiarugi, Anatomist at Florence University, is a peer-reviewed journal sponsored by the Italian Society of Anatomy and Embryology. The journal publishes original papers, invited review articles, historical article, commentaries, obituitary, and book reviews. Its main focus is to understand anatomy through an analysis of structure, function, development and evolution. Priority will be given to studies of that clearly articulate their relevance to the anatomical community. Focal areas include: experimental studies, contributions based on molecular and cell biology and on the application of modern imaging techniques; comparative functional morphology; developmental biology; functional human anatomy; methodological innovations in anatomical research; significant advances in anatomical education. Studies that are essentially descriptive anatomy are appropriate only if they communicate clearly a broader functional or evolutionary significance. All papers should be submitted in English and must be original works that are unpublished and not under consideration by another journal. An international Editorial Board and reviewers from the anatomical disciplines guarantee a rapid review of your paper within two to three weeks after submission.