The Effects of Quanzhen Yiqi Decoction on Inflammatory Response and Immune Function of COPD Rats Were Analyzed Based on LASP1 and CXCR1

Abstract

Objective: To observe the effects of Quanzhen Yiqi Decoction on the expression of LASP1 and CXCR1 in lung tissue of chronic obstructive pulmonary disease (COPD) rats, and to explore the effects of Quanzhen Yiqi Decoction on inflammation and immune function of COPD. Methods: Eighteen SD rats were randomly divided into Chinese medicine group and model group, with 9 rats in each group. COPD rat model was established by smoking combined with lipopolysaccharide (LPS). The model group was given 0.9% normal saline daily. The traditional Chinese medicine group was given 40% Quanzhen Yiqi Decoction concentrated decoction every day. The mrna levels of LASP1 and CXCR1 in rat lung tissue were detected by fluorescence quantitative PCR, and the protein levels of LASP1 and CXCr1 in rat lung tissue were detected by Western blot. Results: The mRNA expression abundance of LASP1 in the Chinese medicine group was 0.145 times that in the model group. The mRNA expression abundance of CXCR1 in the TCM group was 0.614 times of that in the model group. Compared with model group, the protein expression levels of LASP1 and CXCR1 in TCM group were significantly decreased. Conclusions: Quanzhen Yiqi Decoction can inhibit airway inflammation in COPD rats by down-regulating the expressions of LASP1 and CXCR1 genes and proteins, which may be one of the potential mechanisms to reduce the risk of lung cancer caused by COPD.