Cadmium induced changes in antioxidant activity, oxidative damage and andrographolide production in Andrographis paniculata

IF 1.6 3区环境科学与生态学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Chemoecology Pub Date : 2023-08-03 DOI:10.1007/s00049-023-00390-2

Suraj Kumar Mourya, Praveen Mohil, R. Vijayvergia, Anil Kumar

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引用次数: 0

Abstract

Among non-essential heavy metals, cadmium (Cd) pollution in soil continuously increasing by anthropogenic activity such as agriculture fertilizer and industrial sources worldwide and have several harmful impacts on plants and humans. Andrographis paniculata is a highly medicinal plant having diverse range of SMs like andrographolide (AG). The present study was carried out to investigate the effect of Cd on growth, activity of antioxidative enzymes and andrographolide production of A. paniculata. Cd was applied as cadmium chloride (CdCl₂) at 5, 10 and 15 μg g⁻¹ of soil. All the studied treatments of Cd were found to be toxic for A. paniculata. The root-shoot length, root-shoot fresh weight, root-shoot dry weight decreased with increasing concentrations of Cd. Total phenolics and flavonoids content significantly increased with increasing Cd concentration in soil. The activity of catalase (CAT), peroxidase (POX), ascorbate peroxidase (APX), glutathione reductase (GR), guaiacol peroxidase (GPX) and polyphenol oxidase (PPO) increased with increasing concentration of Cd compared to control plants. The maximum activity of CAT, POX, APX, GR, GPX and PPO was observed at 15 µg Cd g⁻¹. The AG content in the leaves of plants increased with Cd application. Maximum AG content was observed at 10 μg g⁻¹ applied dose of Cd. Out of the concentrations studied 5 and 10 μg g⁻¹ were moderately toxic to A. paniculata and produced maximum amount of AG.

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镉对穿心莲抗氧化活性、氧化损伤及穿心莲内酯生成的影响

在非必需重金属中，镉(Cd)污染在全球范围内因农业肥料和工业来源等人为活动而不断增加，并对植物和人类产生多种有害影响。穿心莲(Andrographis paniculata)是一种具有穿心莲内酯(AG)等多种短质成分的药用植物。本试验旨在研究Cd对穿心莲生长、抗氧化酶活性及穿心莲内酯分泌的影响。Cd以氯化镉(CdCl2)的形式施用，浓度分别为5、10和15 μg−1。所研究的所有Cd处理均发现对金针菇有毒性。根冠长、根冠鲜重、根冠干重随Cd浓度的增加而降低，总酚和总黄酮含量随Cd浓度的增加而显著增加。过氧化氢酶(CAT)、过氧化物酶(POX)、抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)、愈创木酚过氧化物酶(GPX)和多酚氧化酶(PPO)活性随Cd浓度的升高而升高。15µg Cd g−1时，CAT、POX、APX、GR、GPX和PPO活性最高。施镉后，植株叶片AG含量增加。在10 μg−1剂量下，银含量最高，其中5和10 μg−1浓度对金针菇有中等毒性，银含量最高。

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来源期刊

Chemoecology 环境科学-生化与分子生物学

CiteScore

4.20

自引率

0.00%

发文量

审稿时长

>36 weeks

期刊介绍： It is the aim of Chemoecology to promote and stimulate basic science in the field of chemical ecology by publishing research papers that integrate evolution and/or ecology and chemistry in an attempt to increase our understanding of the biological significance of natural products. Its scopes cover the evolutionary biology, mechanisms and chemistry of biotic interactions and the evolution and synthesis of the underlying natural products. Manuscripts on the evolution and ecology of trophic relationships, intra- and interspecific communication, competition, and other kinds of chemical communication in all types of organismic interactions will be considered suitable for publication. Ecological studies of trophic interactions will be considered also if they are based on the information of the transmission of natural products (e.g. fatty acids) through the food-chain. Chemoecology further publishes papers that relate to the evolution and ecology of interactions mediated by non-volatile compounds (e.g. adhesive secretions). Mechanistic approaches may include the identification, biosynthesis and metabolism of substances that carry information and the elucidation of receptor- and transduction systems using physiological, biochemical and molecular techniques. Papers describing the structure and functional morphology of organs involved in chemical communication will also be considered.