Molecular Epidemiology of Human Metapneumovirus in Riyadh Province, Saudi Arabia

IF 1.2 Q2 Biochemistry, Genetics and Molecular Biology
H. Amer
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引用次数: 10

Abstract

Human metapneumovirus (HMPV) is an important cause of respiratory tract illness in children. Two HMPV subgroups, A and B, and four genotypes, A1, A2, B1 and B2, have been identified. Concurrent circulation of the different genotypes in yearly epidemics has been recorded globally, but not in Saudi Arabia. The current report was designed to study HMPV epidemiology in Saudi children and to analyze the genetic diversity and circulation patterns. Nasopharyngeal aspirates (n = 174) were collected from hospitalized children in Riyadh (2008-2009). The screening of samples using real-time RT-PCR identified 19 HMPV strains. The majority of the strains belonged to subgroup B, while all strains of subgroup A were members of genotype A2. In 2008, only subgroup B was recognized, whereas in 2009 both subgroups were identified to be cocirculating at similar rates. The full-length attachment (G) gene and a partial sequence of the fusion (F) gene of positive samples were sequenced. The G gene showed a high degree of genetic diversity and exhibited a variable number of positively selected sites in different lineages. In contrast, the F gene demonstrated an extensive genetic stability with a higher tendency toward purifying selection. This is the first report on HMPV genotype circulation in Saudi Arabia; however, the exact circulation kinetics requires further retrospective and prospective study.
沙特阿拉伯利雅得省人偏肺病毒的分子流行病学
人偏肺病毒(HMPV)是儿童呼吸道疾病的重要病因。已确定两个HMPV亚群A和B,以及四个基因型A1、A2、B1和B2。在每年的流行中,不同基因型的同时传播在全球都有记录,但在沙特阿拉伯没有。本报告旨在研究沙特儿童的HMPV流行病学,并分析遗传多样性和传播模式。从利雅得(2008-2009年)住院儿童中收集了鼻咽吸入物(n = 174)。采用实时RT-PCR对样本进行筛选,鉴定出19株HMPV毒株。大多数菌株属于B亚群,而A亚群的所有菌株均为A2基因型成员。在2008年,只有B亚群被确认,而在2009年,两个亚群被确认以相似的速率共同循环。对阳性样品的全长附着(G)基因和融合(F)基因的部分序列进行测序。G基因表现出高度的遗传多样性,在不同的世系中表现出不同数量的正选择位点。相比之下,F基因表现出广泛的遗传稳定性和更高的纯化选择倾向。这是沙特阿拉伯关于HMPV基因型传播的第一份报告;然而,确切的循环动力学需要进一步的回顾性和前瞻性研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Molecular Microbiology and Biotechnology
Journal of Molecular Microbiology and Biotechnology 生物-生物工程与应用微生物
CiteScore
3.90
自引率
0.00%
发文量
0
审稿时长
>12 weeks
期刊介绍: We are entering a new and exciting era of microbiological study and application. Recent advances in the now established disciplines of genomics, proteomics and bioinformatics, together with extensive cooperation between academic and industrial concerns have brought about an integration of basic and applied microbiology as never before.
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